Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima.
Anim Sci J. 2013 Nov;84(11):719-25. doi: 10.1111/asj.12067. Epub 2013 May 1.
In this study, the effects of the addition of L-carnitine in in vitro maturation (IVM) medium for bovine oocytes on their nuclear maturation and cryopreservation were investigated; they were matured in IVM medium supplemented with 0.0, 0.3, 0.6 and 1.2 mg/mL of L-carnitine (control, 0.3, 0.6 and 1.2 groups, respectively) and some of them were vitrified by Cryotop. Moreover, the effects of L-carnitine during in vitro fertilization (IVF) and in vitro culture (IVC) on the developmental potential and quality of IVF embryos were also examined. A significantly higher maturation rate of oocytes was obtained for 0.3 and 0.6 mg/mL groups compared with the control (P < 0.05). The blastocyst formation rate in the 0.6 group was significantly improved, whereas the rate in the 1.2 group was significantly decreased when compared with the control group (P < 0.05). No significant difference was found in embryo development between the control and the L-carnitine group after oocyte vitrification. Supplementation of IVF and IVC media with L-carnitine had no effect on development to the blastocyst stage of IVM oocytes treated with 0.6 mg/mL L-carnitine. In conclusion, the supplementation of L-carnitine during IVM of bovine oocytes improved their nuclear maturation and subsequent embryo development after IVF, but when they were vitrified the improving effects were neutralized.
本研究探讨了在牛卵母细胞体外成熟（IVM）培养基中添加左旋肉碱对其核成熟和冷冻保存的影响；它们在补充了 0.0、0.3、0.6 和 1.2mg/mL 左旋肉碱的 IVM 培养基（对照组、0.3、0.6 和 1.2 组）中成熟，其中一些通过 Cryotop 进行了冷冻。此外，还检查了左旋肉碱在体外受精（IVF）和体外培养（IVC）过程中对 IVF 胚胎发育潜力和质量的影响。与对照组相比，0.3 和 0.6mg/mL 组的卵母细胞成熟率显著提高（P<0.05）。0.6 组的囊胚形成率显著提高，而 1.2 组的囊胚形成率显著降低与对照组相比（P<0.05）。卵母细胞冷冻后，对照组和左旋肉碱组胚胎发育无显著差异。在添加 0.6mg/mL 左旋肉碱的 IVM 卵母细胞中补充 IVF 和 IVC 培养基中的左旋肉碱对其发育至囊胚阶段没有影响。总之，在牛卵母细胞的 IVM 中添加左旋肉碱可提高其核成熟和随后的 IVF 胚胎发育，但当它们被冷冻时，这种改善作用被中和。
