Liukkonen Joonas, Gürsoy Ulvi K, Könönen Eija, Akhi Ramin, Salminen Aino, Liljestrand John M, Pradhan-Palikhe Pratikshya, Pietiäinen Milla, Sorsa Timo, Persson G Rutger, Mäntylä Päivi, Buhlin Kåre, Paju Susanna, Sinisalo Juha, Hörkkö Sohvi, Pussinen Pirkko J
Institute of Dentistry, University of Turku, 20100 Turku, Finland.
Oral Health Care, Welfare Division, City of Turku, 20100 Turku, Finland.
Diagnostics (Basel). 2020 Aug 5;10(8):560. doi: 10.3390/diagnostics10080560.
The cumulative risk score (CRS) is a mathematical salivary diagnostic model to define an individual's risk of having periodontitis. In order to further validate this salivary biomarker, we investigated how periodontal bacteria, lipopolysaccharide (LPS), and systemic and local host immune responses relate to CRS. Subgingival plaque, saliva, and serum samples collected from 445 individuals were used in the analyses. Plaque levels of 28 microbial species, especially those of , , , , and , and serum and salivary levels of IgA and IgG against these five species were determined. Additionally, LPS activity was measured. High CRS associated strongly with all IgA/IgG antibody and LPS levels in saliva, whereas in serum the associations were not that obvious. In the final logistic regression model, the best predictors of high CRS were saliva IgA burden against the five species (OR 7.04, 95% CI 2.25-22.0), IgG burden (3.79, 1.78-8.08), LPS (2.19, 1.38-3.47), and the sum of 17 subgingival Gram-negative species (6.19, 2.10-18.3). CRS is strongly associated with microbial biomarker species of periodontitis and salivary humoral immune responses against them.
累积风险评分（CRS）是一种数学唾液诊断模型，用于定义个体患牙周炎的风险。为了进一步验证这种唾液生物标志物，我们研究了牙周细菌、脂多糖（LPS）以及全身和局部宿主免疫反应与CRS之间的关系。分析中使用了从445名个体收集的龈下菌斑、唾液和血清样本。测定了28种微生物的菌斑水平，尤其是 、 、 、 和 的菌斑水平，以及针对这五种菌种的血清和唾液中IgA和IgG的水平。此外，还测量了LPS活性。高CRS与唾液中所有IgA/IgG抗体和LPS水平密切相关，而在血清中这种相关性并不那么明显。在最终的逻辑回归模型中，高CRS的最佳预测指标是唾液中针对这五种菌种的IgA负荷（比值比7.04，95%置信区间2.25 - 22.0）、IgG负荷（3.79，1.78 - 8.08）、LPS（2.19，1.38 - 3.47）以及17种龈下革兰氏阴性菌种的总和（6.19，2.10 - 18.3）。CRS与牙周炎的微生物生物标志物菌种以及针对它们的唾液体液免疫反应密切相关。
