Department of Cardiovascular Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China (mainland).
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China (mainland).
Med Sci Monit. 2020 Aug 7;26:e922986. doi: 10.12659/MSM.922986.
BACKGROUND We aimed to investigate the effects of norepinephrine (NE) released from endogenous stores on bacterial translocation of Escherichia coli in mice by administration of 6-hydroxydopamine (6-OHDA), which selectively destroys noradrenergic nerve terminals. MATERIAL AND METHODS E. coli strain BW25113 and its derivatives (BW25113ΔqseC and BW25113ΔqseC pQseC) were used in this study. The serum concentrations of endotoxin were analyzed. The strains BW25113, BW25113ΔqseC, and BW25113ΔqseC pQseC were detected respectively in tissue specimens harvested from mice treated with 6-OHDA. RESULTS Mice treated with BW25113ΔqseC showed reduced levels of bacterial translocation following administration of 6-OHDA compared with mice treated with BW25113. The defect of E. coli QseC receptor caused the norepinephrine-QseC signal chain to be interrupted, and the invasiveness and penetrating power of the bacteria on the intestinal mucosa was weakened, eventually leading to a significant decrease in the incidence of bacterial translocation. CONCLUSIONS NE modulates the interaction of enteric bacterial pathogens with their hosts via QseC. The blockade of the QseC receptor-mediated effects may be useful to attenuate bacterial translocation.
我们旨在通过给予 6-羟多巴胺（6-OHDA）来研究内源性储存的去甲肾上腺素（NE）对小鼠大肠杆菌细菌易位的影响，6-OHDA 可选择性地破坏去甲肾上腺素能神经末梢。
本研究使用了大肠杆菌 BW25113 及其衍生物（BW25113ΔqseC 和 BW25113ΔqseC pQseC）。分析了血清内毒素浓度。在接受 6-OHDA 治疗的小鼠组织标本中检测了 BW25113、BW25113ΔqseC 和 BW25113ΔqseC pQseC 这三种菌株。
与接受 BW25113 治疗的小鼠相比，接受 BW25113ΔqseC 治疗的小鼠在给予 6-OHDA 后细菌易位水平降低。大肠杆菌 QseC 受体的缺陷导致去甲肾上腺素-QseC 信号链被中断，细菌对肠黏膜的侵袭力和穿透力减弱，最终导致细菌易位的发生率显著降低。
NE 通过 QseC 调节肠道细菌病原体与其宿主的相互作用。阻断 QseC 受体介导的作用可能有助于减轻细菌易位。
