Wang Yiru, Jia Qingyun, Zhang Yifan, Wei Jing, Liu Ping
Department of Cardiology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Second Ward of Trauma Surgery Department, Linyi People's Hospital, Linyi, China.
Front Pharmacol. 2020 Jul 17;11:1070. doi: 10.3389/fphar.2020.01070. eCollection 2020.
Taoren Honghua drug is a traditional Chinese medicinal drug used to treat cardiovascular disease. The aim of the study is to investigate the effects of Taoren Honghua drug on inflammation and atherosclerosis in ApoE knock-out mice and RAW264.7 cells. ApoE knock-out mice fed with high fat diet for 8 weeks were randomly divided into five groups and then continued the high fat diet, or plus Taoren Honghua drug at concentrations of 3.63, 1.815, and 0.9075 g/ml, or plus Simvastatin at 2.57 mg/kg. RAW 264.7 cells were intervened with lipopolysaccharide or lipopolysaccharide plus different concentrations of Taoren Honghua drug. Compared to mice only with high fat diet, mice with high fat diet and Taoren Honghua drug showed lower body weight, triglyceride, cholesterol, IL-6 and TNF-α, smaller plaque sizes, less lymph vessel, and T cell contents of lymph nodes, but higher IL-10 level. In RAW264.7 cells, groups with LPS plus Taoren Honghua drug had lower IL-6 and TNF-α, but higher IL-10 than LPS group, as revealed by PCR or ELISA methods. A decrease of total or phosphorylated ERK1/2, JNK, p38, ERK5, STAT3, and AKT were detected, so was the translocation of NF-κB p65 from nuclear to cytoplasm. These results suggested that Taoren Honghua drug could attenuate atherosclerosis and play an anti-inflammatory role MAPKs, ERK5/STAT3, and AKT/NF-κB p65 signaling pathways in ApoE knock-out mice and lipopolysaccharide-induced RAW264.7 cells.
桃仁红花药是一种用于治疗心血管疾病的传统中药。本研究的目的是探讨桃仁红花药对载脂蛋白E基因敲除小鼠和RAW264.7细胞炎症及动脉粥样硬化的影响。将高脂饮食喂养8周的载脂蛋白E基因敲除小鼠随机分为五组，然后继续高脂饮食，或分别添加浓度为3.63、1.815和0.9075 g/ml的桃仁红花药，或添加2.57 mg/kg的辛伐他汀。用脂多糖或脂多糖加不同浓度的桃仁红花药干预RAW 264.7细胞。与仅高脂饮食的小鼠相比，高脂饮食加桃仁红花药的小鼠体重、甘油三酯、胆固醇、白细胞介素-6和肿瘤坏死因子-α较低，斑块尺寸较小，淋巴管较少，淋巴结T细胞含量较少，但白细胞介素-10水平较高。在RAW264.7细胞中，脂多糖加桃仁红花药组的白细胞介素-6和肿瘤坏死因子-α低于脂多糖组，但白细胞介素-10高于脂多糖组，这通过聚合酶链反应或酶联免疫吸附测定法得以揭示。检测到总细胞外信号调节激酶1/2、应激活化蛋白激酶、p38丝裂原活化蛋白激酶、细胞外信号调节激酶5、信号转导和转录激活因子3以及蛋白激酶B的减少，核因子-κB p65从细胞核向细胞质的转位也减少。这些结果表明，桃仁红花药可减轻动脉粥样硬化，并在载脂蛋白E基因敲除小鼠和脂多糖诱导的RAW264.7细胞中通过丝裂原活化蛋白激酶、细胞外信号调节激酶5/信号转导和转录激活因子3以及蛋白激酶B/核因子-κB p65信号通路发挥抗炎作用。
