Advancecor GmbH, Lochhamerstr. 29 A, 82152, Martinsried, Germany.
Sci Rep. 2022 Aug 3;12(1):13342. doi: 10.1038/s41598-022-17380-z.
Histological examination of targets in regions of interest in histological sections is one of the most frequently used tools in biomedical research. However, it is a technical challenge to secure a multitarget section for inspection of the structure's mutual relationship of targets or a longitudinally filamentous- or tubular-formed tissue section for visitation of the overall morphological features. We present a method with a specified cutting plane and place, allowing researchers to cut directly at the multitarget centers accurately and quickly. The method is proven to be reliable with high accuracy and reproducibility and a low coefficient of variation, testing on repeat experiments of three target's position-known models. With this method, we successfully yielded single sections containing whole intraorbital optical nerves, three aortic valves, or whole thoracic tracheas in their central positions. The adjoined custom-made tools used in the study, such as various tissue-specific formulated calibrated trimming and embedding guides, an organ-shaped cavity plaster mold, and a two-time embedding technique for optimal and identical trimming or embedding, also bear great potential to become a common supplemental tool for traditional histology and may contribute to the reduction of the labor, and the number of animals needed.
在组织学切片的感兴趣区域进行目标的组织学检查是生物医学研究中最常用的工具之一。然而，对于需要检查目标相互关系的多目标切片，或者需要检查整体形态特征的纵向丝状或管状组织切片，确保安全是一个技术挑战。我们提出了一种具有特定切割平面和位置的方法，允许研究人员准确快速地直接在多目标中心进行切割。该方法经过三个已知目标位置模型的重复实验测试，证明具有高精度和可重复性，且变异系数低。使用该方法，我们成功地获得了包含完整眶内视神经、三个主动脉瓣或整个胸气管的中央位置的单个切片。研究中使用的附加定制工具，如各种组织特异性校准修整和包埋指南、器官形状腔石膏模具，以及两次包埋技术以实现最佳和相同的修整或包埋，也具有成为传统组织学的常用补充工具的潜力，并有助于减少劳动力和所需动物的数量。
