García-Fontana Cristina, Vilchez Juan Ignacio, Manzanera Maximino
Institute for Water Research and Department of Microbiology, University of Granada, Granada, Spain.
Front Microbiol. 2020 Jul 10;11:1537. doi: 10.3389/fmicb.2020.01537. eCollection 2020.
Desiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for improving drought tolerance in crops. This understanding is especially relevant in an environment that tends to increase the number and intensity of droughts. The combination of certain microorganisms with drought-sensitive plants can improve their tolerance to water scarcity. One of these bacteria is sp. 3J1, an actinobacteria able to protect pepper plants from drought. In this study, we supplemented drought-tolerant and drought-sensitive plant rhizospheres with sp. 3J1 and analyzed their proteomes under drought to investigate the plant-microbe interaction. We also compare this root proteome with the proteome found in desiccation-tolerant plants. In addition, we studied the proteome of sp. 3J1 subjected to drought to analyze its contribution to the plant-microbe interaction. We describe those mechanisms shared by desiccation-tolerant plants and sensitive plants protected by microorganisms focusing on protection against oxidative stress, and production of compatible solutes, plant hormones, and other more specific proteins.
耐旱植物能够在缺水的情况下存活很长时间。从分子层面了解这些植物抵御干旱的机制，对于提高作物的耐旱性具有重要价值。在干旱次数和强度趋于增加的环境中，这种认识尤为重要。某些微生物与干旱敏感型植物相结合，可以提高它们对缺水的耐受性。其中一种细菌是sp. 3J1，这是一种能够保护辣椒植株免受干旱影响的放线菌。在本研究中，我们用sp. 3J1补充耐旱和干旱敏感型植物的根际，并分析它们在干旱条件下的蛋白质组，以研究植物与微生物的相互作用。我们还将这种根系蛋白质组与耐旱植物中的蛋白质组进行比较。此外，我们研究了遭受干旱的sp. 3J1的蛋白质组，以分析其对植物与微生物相互作用的贡献。我们描述了耐旱植物和受微生物保护的敏感植物共有的那些机制，重点是针对氧化应激的保护以及相容性溶质、植物激素和其他更特殊蛋白质的产生。
