Caulfield J P, Yuan H C, Cianci C M, Hein A
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts.
Exp Parasitol. 1988 Feb;65(1):10-9. doi: 10.1016/0014-4894(88)90102-6.
The development of the cercarial glycocalyx of Schistosoma mansoni was studied by transmission electron microscopy and immunofluorescence light microscopy employing antibodies raised against extracted and chromatographed glycocalyx. By electron microscopy, cercariae present in the brood chamber of daughter sporocysts were surrounded by an electron-dense granular and fibrillar matrix. This material appeared structurally distinct from the glycocalyx which was coarsely fibrillar and located only on the surface of organisms that had developed a final tegument. The thickness of the glycocalyx apparently increased with the maturation of the tegument, since teguments that had many spines also had the thickest glycocalyx. Immunofluorescent staining of frozen sections of infected snail hepatopancreas showed that glycocalyx antigens were present on the surface of the cercariae and not in the matrix within the brood chamber or in snail tissues. Immunofluorescent staining of isolated larval cercariae showed staining of some but not all parasites with partially elongated tails. These studies suggest that the glycocalyx develops late in cercarial development (late in Stage 6 or in Stage 7 of Cheng and Bier), is made by the cercariae themselves, and is not a product of either the sporocyst wall cells or snail hepatopancreas.
利用针对提取并经色谱分析的糖萼制备的抗体，通过透射电子显微镜和免疫荧光光学显微镜研究了曼氏血吸虫尾蚴糖萼的发育情况。通过电子显微镜观察，子胞蚴育囊内的尾蚴被电子致密的颗粒状和纤维状基质所包围。这种物质在结构上与糖萼不同，糖萼呈粗纤维状，仅位于已发育出最终皮层的生物体表面。糖萼的厚度显然随着皮层的成熟而增加，因为有许多棘的皮层也有最厚的糖萼。对感染蜗牛肝胰腺冰冻切片的免疫荧光染色显示，糖萼抗原存在于尾蚴表面，而不存在于育囊内的基质或蜗牛组织中。对分离出的幼虫尾蚴的免疫荧光染色显示，部分尾巴已部分伸长的一些但并非所有寄生虫都有染色。这些研究表明，糖萼在尾蚴发育后期（Cheng和Bier的第6阶段后期或第7阶段）形成，由尾蚴自身产生，而非孢囊壁细胞或蜗牛肝胰腺的产物。
