Chen Wei-da, Song Ting, Cao Qiu-Hong, Li Rui, Wang Hua, Chen Xiu-Bao, Chen Ze-Tao
Health Care Department, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, P.R. China.
Health Care Department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, P.R. China.
Exp Ther Med. 2020 Sep;20(3):2863-2869. doi: 10.3892/etm.2020.9025. Epub 2020 Jul 21.
Using a series of DNA methylation analysis, pathogenesis was investigated to identify the specific DNA methylation markers for diagnosing atherosclerosis. Firstly, with the chip platform of Illumina Human Methylation 450 BeadChip, a total of 1,458 CpGs, covering 971 differential methylated genes were extracted with stringent filtering criteria. Secondly, hierarchical clustering as a heat map was used to check on the dependability of differential methylated genes. Thirdly, the related GO terms and pathways were enriched by up- and down-methylated genes, respectively, after verifying the capacity of these differential methylated genes to distinguish between atherosclerosis and healthy controls. In total, 971 differential DNA methylated genes were identified (1,458 CpGs). Several important function regions were also identified, including cell adhesion, PI3K-Akt signaling pathway and transcription from RNA polymerase II promoter. This study indicates that patients with atherosclerosis have high levels of DNA methylation, which is promising for early diagnosis and treatment of atherosclerosis.
通过一系列DNA甲基化分析，对动脉粥样硬化的发病机制进行了研究，以确定用于诊断动脉粥样硬化的特定DNA甲基化标记物。首先，利用Illumina Human Methylation 450 BeadChip芯片平台，按照严格的筛选标准，共提取了1458个覆盖971个差异甲基化基因的CpG位点。其次，使用层次聚类作为热图来检查差异甲基化基因的可靠性。第三，在验证这些差异甲基化基因区分动脉粥样硬化和健康对照的能力后，分别通过甲基化上调和下调的基因对相关的基因本体（GO）术语和信号通路进行富集。总共鉴定出971个差异DNA甲基化基因（145 CpG位点）。还确定了几个重要的功能区域，包括细胞黏附、PI3K-Akt信号通路和RNA聚合酶II启动子的转录。这项研究表明，动脉粥样硬化患者的DNA甲基化水平较高，这对于动脉粥样硬化的早期诊断和治疗具有重要意义。
