Ly Bui Thi Kim, Chi Hoang Thanh
Department of Food Technology, Ho Chi Minh City University of Technology Institute of Applied Sciences, Ho Chi Minh City University of Technology, Ho Chi Minh 72308, Vietnam.
Southern Key Laboratory of Biotechnology, Institute of Fungal Research and Biotechnology, Hanoi 12000, Vietnam.
Biomed Rep. 2020 Oct;13(4):25. doi: 10.3892/br.2020.1332. Epub 2020 Jul 22.
Patents diagnosed with acute promyelocytic leukemia were treated with Vesanoid [all-trans retinoic acid (ATRA)]. ATRA promotes the maturation and differentiation of leukemia cells and is therefore capable of reducing the symptoms of leukemia by preventing aggregation of myeloid cells. However, the clinical applications of ATRA are limited by its side effects, including acute retinoid resistance, hypertriglyceridemia, mucocutaneous dryness, nausea, brief recovery time relapse and drug resistance. Therefore, combinations of ATRA and other anticancer drugs are being investigated to overcome these limitations. In our previous study it was shown that in leukemia cells, (-)-epigallocatechin-3-gallate (EGCG) reduced cell proliferation and induced apoptotic cell death. In the present study, an evaluation of the effects of the combination of EGCG and ATRA on -mutated cell lines was performed using the isobologram method. The results showed that there was an additive effect in leukemic cells when treated with a combination of ATRA and EGCG. Thus, it was concluded that the cytotoxic effects of EGCG were improved by ATRA.
被诊断为急性早幼粒细胞白血病的患者用维甲酸（全反式维甲酸，ATRA）进行治疗。全反式维甲酸可促进白血病细胞的成熟和分化，因此能够通过防止髓细胞聚集来减轻白血病症状。然而，全反式维甲酸的临床应用受到其副作用的限制，这些副作用包括急性维甲酸抵抗、高甘油三酯血症、皮肤黏膜干燥、恶心、短暂缓解期复发和耐药性。因此，正在研究全反式维甲酸与其他抗癌药物的联合使用，以克服这些局限性。在我们之前的研究中表明，在白血病细胞中，（-）-表没食子儿茶素-3-没食子酸酯（EGCG）可减少细胞增殖并诱导凋亡性细胞死亡。在本研究中，使用等效线图法评估了EGCG与全反式维甲酸联合对突变细胞系的影响。结果表明，当用全反式维甲酸和EGCG联合处理时，白血病细胞中有相加作用。因此，得出结论：全反式维甲酸可增强EGCG的细胞毒性作用。
