Department of ENT and HN Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Eur Rev Med Pharmacol Sci. 2020 Aug;24(15):8001-8007. doi: 10.26355/eurrev_202008_22483.
To elucidate the promotive role of TRPP2 in nasopharyngeal carcinoma (NPC) proliferation by targeting Skp2/c-Myc, thus accelerating the malignant progression.
TRPP2 levels in NPC patients with different T stages were detected. Correlation between TRPP2 level and clinical features of NPC patients was analyzed. Kaplan-Meier curves were depicted for assessing the prognostic value of TRPP2 in NPC. Subsequently, regulatory effects of TRPP2 on viability and 5-ethynyl-2'-deoxyuridine (EdU)-positive ratio were determined by cell counting kit-8 (CCK-8) and EdU assay, respectively. Relative levels of Skp2 and c-Myc in NPC cells transfected with si-TRPP2 were examined. At last, the involvement of c-Myc in TRPP2-regulated proliferative ability of NPC was evaluated by performing rescue experiments.
TRPP2 was upregulated in NPC tissues. TRPP2 level was higher in NPC patients with T3+T4 than those with T1+T2. Worse survival was observed in NPC patients expressing high level of TRPP2. TRPP2 level was correlated to T stage, N stage, M stage, and locoregional failure of NPC patients. Knockdown of TRPP2 reduced viability and EdU-positive ratio in NPC cells. In addition, relative levels of Skp2 and c-Myc in NPC cells transfected with si-TRPP2 were downregulated. Overexpression of c-Myc could partially reverse the regulatory effects of TRPP2 on NPC proliferation.
TRPP2 stimulates NPC cells to proliferate by upregulating expressions of Skp2/c-Myc, thus deteriorating the development of NPC.
通过靶向 Skp2/c-Myc，阐明 TRPP2 在鼻咽癌（NPC）增殖中的促进作用，从而加速恶性进展。
检测不同 T 分期 NPC 患者中 TRPP2 的水平。分析 TRPP2 水平与 NPC 患者临床特征的相关性。绘制 Kaplan-Meier 曲线评估 TRPP2 在 NPC 中的预后价值。随后，通过细胞计数试剂盒-8（CCK-8）和 EdU 检测分别确定 TRPP2 对细胞活力和 EdU 阳性率的调节作用。转染 si-TRPP2 的 NPC 细胞中 Skp2 和 c-Myc 的相对水平。最后，通过进行挽救实验评估 c-Myc 在 TRPP2 调节的 NPC 增殖能力中的参与情况。
TRPP2 在 NPC 组织中上调。TRPP2 水平在 T3+T4 期 NPC 患者中高于 T1+T2 期患者。表达高水平 TRPP2 的 NPC 患者的生存情况较差。TRPP2 水平与 NPC 患者的 T 分期、N 分期、M 分期和局部区域失败有关。敲低 TRPP2 降低了 NPC 细胞的活力和 EdU 阳性率。此外，转染 si-TRPP2 的 NPC 细胞中 Skp2 和 c-Myc 的相对水平下调。c-Myc 的过表达可以部分逆转 TRPP2 对 NPC 增殖的调节作用。
TRPP2 通过上调 Skp2/c-Myc 的表达来刺激 NPC 细胞增殖，从而恶化 NPC 的发展。
