Assimakopoulou Martha, Soufli Konstantina, Melachrinou Maria
Department of Anatomy, Histology and Embryology, School of Medicine, University of Patras, 26504 Patras, Greece.
Department of Pathology, School of Medicine, University of Patras, 26504 Patras, Greece.
Biomedicines. 2025 Apr 5;13(4):884. doi: 10.3390/biomedicines13040884.
Polycystin 1 (PC1) and polycystin 2 (PC2) proteins are members of the transient receptor potential (TRP) channels family and are encoded from and genes, respectively. Until recently, the role of and has been associated with the pathogenesis of the kidney since mutations in these genes cause autosomal dominant polycystic kidney disease (ADPKD). Recent data implicates polycystins in the pathogenesis of solid tumors. In this aspect, the expression of and in human astrocytomas is largely unknown. The aim of the present research study was to investigate the expression of and in astrocytic tumors and correlate it with clinicopathological characteristics such as the grade of malignancy, age, and gender of the patients. : A total of 70 cases-corresponding to 8 grade II (diffuse fibrillary astrocytomas), 12 grade III (anaplastic astrocytomas), and 50 grade IV (glioblastomas multiforme)-were examined. The mRNA expression levels of and were determined through molecular qRT-PCR analysis using the relative quantification ΔΔCt method and the expression of PC1 and PC2 was detected through immunohistochemistry using the semi-quantitative H-score system. : Increased levels of and in astrocytomas were found compared with that of a normal brain ( < 0.05). Glioblastomas demonstrated the greatest increase in and expression compared to other grades of malignancy ( < 0.05). The same pattern of expression showed PC1 and PC2 proteins. A significant correlation between and as well as PC1 and PC2 expressions was found ( < 0.05). Although no association was detected between PC1 or PC2 and Ki67 expression ( > 0.05), a significant correlation between PC1 and p53 immunoexpressions, in grade III and between PC2 and p53 immunoexpressions, in grade II astrocytomas ( < 0.01) has emerged. PC1 expression was correlated with age of the patients ( < 0.05). and expression were negatively correlated with the prognosis of glioma patients. : The results of this study indicate the potential involvement of polycystins in the pathogenesis of astrocytomas. However, further research is required to fully understand the mechanisms that these molecules are implicated.
多囊蛋白1（PC1）和多囊蛋白2（PC2）是瞬时受体电位（TRP）通道家族的成员，分别由 和 基因编码。直到最近， 和 的作用一直与肾脏发病机制相关，因为这些基因的突变会导致常染色体显性多囊肾病（ADPKD）。最近的数据表明多囊蛋白与实体瘤的发病机制有关。在这方面， 和 在人类星形细胞瘤中的表达情况 largely unknown。本研究的目的是调查 和 在星形细胞瘤中的表达，并将其与临床病理特征（如恶性程度、患者年龄和性别）相关联。：共检查了70例病例，对应8例二级（弥漫性纤维性星形细胞瘤）、12例三级（间变性星形细胞瘤）和50例四级（多形性胶质母细胞瘤）。 和 的mRNA表达水平通过使用相对定量ΔΔCt方法的分子qRT-PCR分析来确定，PC1和PC2的表达通过使用半定量H评分系统的免疫组织化学检测。：与正常脑组织相比，星形细胞瘤中 和 的水平升高（<0.05）。与其他恶性程度等级相比，多形性胶质母细胞瘤中 和 的表达增加最为明显（<0.05）。PC1和PC2蛋白也呈现相同的表达模式。发现 和 以及PC1和PC2的表达之间存在显著相关性（<0.05）。虽然未检测到PC1或PC2与Ki67表达之间的关联（>0.05），但在三级星形细胞瘤中PC1与p53免疫表达之间以及在二级星形细胞瘤中PC2与p53免疫表达之间出现了显著相关性（<0.01）。PC1的表达与患者年龄相关（<0.05）。 和 的表达与胶质瘤患者的预后呈负相关。：本研究结果表明多囊蛋白可能参与星形细胞瘤的发病机制。然而，需要进一步研究以充分了解这些分子所涉及的机制。
