Santos F D S, Ferreira M R A, Maubrigades L R, Gonçalves V S, de Lara A P S, Moreira C, Salvarani F M, Conceição F R, Leivas Leite F P
Center for Technological Development, Postgraduate Program in Biotechnology, Federal University of Pelotas, Pelotas, Brazil.
Institute of Biology, Postgraduate Program in Parasitology, Federal University of Pelotas, Pelotas, Brazil.
J Appl Microbiol. 2021 Mar;130(3):699-706. doi: 10.1111/jam.14814. Epub 2020 Aug 25.
The aim of the present study was to examine the vaccine immune response in ewes supplemented with Bacillus toyonensis BCT-7112 during a period of 5-day supplementation before vaccination against a recombinant Clostridium perfringens epsilon toxin (rETX).
Ewes were vaccinated with 200 µg of rETX adjuvanted with 10% aluminium hydroxide. The treat group was orally supplemented with B. toyonensis BCT-7112 (3 × 10 viable spores) for 5 days prior to the first and second vaccination. Ewes supplemented with B. toyonensis BCT-7112 showed higher neutralizing antibody titres than the non-supplemented ewes (P < 0·05), with an increase in serum levels for total IgG anti-rETX by 3·2-fold (P < 0·0001), and for both IgG isotypes IgG1 and IgG2 by 2·1-fold and 2·3-fold (P < 0·01), respectively, compared with the control group. The peripheral blood mononuclear cells of ewes in the supplemented group had a higher (P < 0·05) cytokine mRNA transcription levels for IL-2 (6·4-fold increase), IFN-γ (2·9-fold increase) and transcription factor Bcl6 (2·3-fold increase) compared with the control group.
We conclude that a 5 days of supplementation with B. toyonensis BCT-7112 prior vaccination is sufficient to significantly improve the humoral immune response of ewes against C. perfringens recombinant ETX vaccine.
These findings open a new perspective in the utilization of B. toyonensis BCT-7112 as an immunomodulator since a 5 days period of probiotic supplementation is sufficient to improve the vaccine immune response.
本研究旨在检测在接种重组产气荚膜梭菌ε毒素（rETX）疫苗前5天补充toyonensis芽孢杆菌BCT - 7112的母羊的疫苗免疫反应。
用200μg佐以10%氢氧化铝的rETX对母羊进行接种。治疗组在第一次和第二次接种前5天口服补充toyonensis芽孢杆菌BCT - 7112（3×10个活孢子）。补充toyonensis芽孢杆菌BCT - 7112的母羊比未补充的母羊表现出更高的中和抗体滴度（P<0.05），与对照组相比，抗rETX的总IgG血清水平增加了3.2倍（P<0.0001），IgG1和IgG2两种IgG亚型分别增加了2.1倍和2.3倍（P<0.01）。与对照组相比，补充组母羊的外周血单个核细胞中IL - 2（增加6.4倍）、IFN - γ（增加2.9倍）和转录因子Bcl6（增加2.3倍）的细胞因子mRNA转录水平更高（P<0.05）。
我们得出结论，在接种疫苗前5天补充toyonensis芽孢杆菌BCT - 7112足以显著改善母羊对产气荚膜梭菌重组ETX疫苗的体液免疫反应。
这些发现为将toyonensis芽孢杆菌BCT - 7112用作免疫调节剂开辟了新的视角，因为5天的益生菌补充期足以改善疫苗免疫反应。
