Roos Talita Bandeira, de Moraes Carina Martins, Sturbelle Régis Tuchtenhagen, Dummer Luana Alves, Fischer Geferson, Leite Fábio Pereira Leivas
Programa de Pós-Graduação em Saúde Animal na Amazônia, Universidade Federal do Pará, Brazil.
Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Brazil.
Res Vet Sci. 2018 Apr;117:260-265. doi: 10.1016/j.rvsc.2017.12.022. Epub 2017 Dec 28.
There have been significant efforts toward the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotics Bacillus toyonensis and Saccharomyces boulardii as a potential immune modulator to improve vaccine efficiency. Thirty, 5-month-old lambs were randomly grouped in three lots of 10 each and vaccinated at days 0, 21 and 42 of the experiment. They grazed on the same pasture and were fed ad libitum twice a day with commercial sheep feed supplemented with either B. toyonensis (1×10CFU/g of feed) or S. boulardii (1×10CFU/g of feed), or non-supplemented feed. The probiotic supplementation was suspended day 28; thereafter, the next 35days, they were fed with the same commercial feed as control group. Animals supplemented with probiotics showed a significant (p>0.001) increased seroconversions against BoHV-5, and higher neutralizing antibodies titres (p>0.05) to BoHV-5 than non-supplemented animals. At 63days of experiment, splenocytes from the supplemented sheep had higher mRNA transcription levels of cytokines IL-10 and IL-17A. These results suggest that these probiotics could provide a promising means of improving vaccine efficacy.
为开发更有效的动物健康疫苗已付出了巨大努力。一种可用于提高疫苗效力的策略是使用益生菌。牛疱疹病毒5型（BoHV - 5）是一种重要的动物病原体，针对它的疫苗仅提供了有限的保护。在本研究中，我们研究了将益生菌toyonensis芽孢杆菌和布拉迪酵母作为潜在的免疫调节剂以提高疫苗效率。30只5月龄羔羊被随机分成3组，每组10只，并在实验的第0、21和42天进行接种。它们在同一牧场放牧，每天两次随意喂食添加了toyonensis芽孢杆菌（1×10CFU/克饲料）或布拉迪酵母（1×10CFU/克饲料）的商业羊饲料，或未添加的饲料。益生菌补充在第28天暂停；此后的35天，它们被喂食与对照组相同的商业饲料。补充了益生菌的动物针对BoHV - 5的血清转化率显著提高（p>0.001），并且对BoHV - 5的中和抗体滴度高于未补充的动物（p>0.05）。在实验的第63天，补充了益生菌的绵羊的脾细胞中细胞因子IL - 10和IL - 17A的mRNA转录水平更高。这些结果表明，这些益生菌可能为提高疫苗效力提供一种有前景的方法。
