Bird C R, Gearing A J, Thorpe R
National Institute for Biological Standards and Control, Herts, U.K.
J Immunol Methods. 1988 Feb 10;106(2):175-9. doi: 10.1016/0022-1759(88)90194-9.
The use of Tween 20 as a suitable blocking agent in immunoblotting studies was evaluated by screening a panel of monoclonal antibodies (MoAbs) against a selection of blotted proteins which were unrelated to the antigens used to raise the MoAbs. Using Tween 20 alone to block the nitrocellulose membranes clear reactions were observed between the panel of MoAbs and several components of the blotted protein mixture. In contrast, when haemoglobin was used to block the membranes such reactions were not observed. In the absence of added protein the use of Tween 20 alone as a blocking agent for immunoblotting appears to lead to false positive reactions by non-specific antigen-antibody complexes.
通过筛选一组针对一系列与用于制备单克隆抗体（MoAbs）的抗原无关的印迹蛋白的单克隆抗体，评估了吐温20作为免疫印迹研究中合适封闭剂的用途。仅使用吐温20封闭硝酸纤维素膜时，观察到该组单克隆抗体与印迹蛋白混合物的几种成分之间发生了明显反应。相比之下，当使用血红蛋白封闭膜时，未观察到此类反应。在不添加蛋白质的情况下，仅使用吐温20作为免疫印迹的封闭剂似乎会导致非特异性抗原-抗体复合物产生假阳性反应。
