Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain; Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain; Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain; Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT) (CONICET-Universidad Nacional de Córdoba) ICTA & Cátedra de Química Biológica Departamento de Química FCEFyN, UNC. Av. Velez Sarsfield 1611, X 5016GCA Córdoba, Argentina.
J Control Release. 2020 Nov 10;327:61-69. doi: 10.1016/j.jconrel.2020.08.007. Epub 2020 Aug 5.
Growth factors are required for cell proliferation and differentiation under physiological conditions but especially in the context of regenerative medicine. The time-prolonged administration of those factors has been explored using different sustained drug delivery systems. These platforms include natural materials such as bacterial inclusion bodies (IBs) that contain chaperones and other bacterial components that might favour protein release. Being successful from a functional point of view, IBs pose regulatory concerns to clinical applications because of the mentioned presence of bacterial cell components, including endotoxins. We have here explored the release and activity of the human fibroblast growth factor-2 (hFGF-2) from a novel synthetic material, namely artificial IBs. Being chemically homogenous and compliant with regulatory restrictions, we wondered if these materials would effectively release functional proteins in absence of accompanying bacterial agents. The data provided here fully supports that artificial hFGF-2 IBs act as true and efficient secretory granules and they slowly disintegrate in cell culture to promote wound healing in an in vitro wound healing model. Free from undesired bacterial components, artificial inclusion bodies show promises as delivery agents in regenerative medicine.
在生理条件下，生长因子是细胞增殖和分化所必需的，但在再生医学的背景下尤其如此。已经探索了使用不同的持续药物输送系统来延长这些因子的给药时间。这些平台包括天然材料，如包含伴侣蛋白和其他可能有利于蛋白质释放的细菌成分的细菌包涵体 (IBs)。从功能角度来看，IBs 是成功的，但由于存在细菌细胞成分，包括内毒素，它们对临床应用存在监管方面的担忧。在这里，我们探索了新型合成材料即人工 IBs 中人类成纤维细胞生长因子-2 (hFGF-2) 的释放和活性。由于化学均一且符合监管限制，我们想知道这些材料是否在没有伴随的细菌剂的情况下能有效地释放功能性蛋白质。这里提供的数据完全支持人工 hFGF-2 IBs 作为真正有效的分泌颗粒发挥作用，并在细胞培养中缓慢崩解，以在体外伤口愈合模型中促进伤口愈合。由于没有不需要的细菌成分，人工包涵体有望成为再生医学中的递送剂。
