Tang Ronggui, Lan Ping, Ding Changfeng, Wang Junsong, Zhang Taolin, Wang Xingxiang
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, 311300, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
J Hazard Mater. 2020 Nov 5;398:122825. doi: 10.1016/j.jhazmat.2020.122825. Epub 2020 May 20.
The toxicity of low-level arsenic (As)-contaminated soil is not well understood. An integrated proteomic and metabolomic approach combined with morphological examination was used to investigate the potential biological toxicity of As-contaminated soil based on an exposure experiment with the earthworm Eisenia fetida. The results showed that the earthworm hindgut accumulated high As concentrations resulting in injury to the intestinal epithelia, chloragogenous tissues and coelom tissues. Furthermore, As-contaminated soil induced a significant increase in betaine levels and a decrease in dimethylglycine and myo-inositol levels in the earthworms, suggesting that the osmoregulatory metabolism of the earthworms may have been disturbed. The significantly altered levels of asparagine and dimethylglycine were proposed as potential biomarkers of As-contaminated soil. The upregulation of soluble calcium-binding proteins and profilin, the downregulation of sodium/potassium-transporting ATPase, and the proteins changes identified by gene ontology enrichment analysis confirmed that the earthworms suffered from osmotic stress. In addition, the significant changes in glycine-tRNA ligase activity and coelomic tissue injury revealed that As accumulation may disturb the earthworm immune system. This work provided new insight into the proteomic and metabolic toxicity of low-level As-contaminated soil ecosystems in earthworms, extended our knowledge of dual omics and highlighted the mechanisms underlying toxicity.
低水平砷（As）污染土壤的毒性尚未得到充分了解。基于对赤子爱胜蚓的暴露实验，采用蛋白质组学和代谢组学相结合的方法并结合形态学检查，来研究砷污染土壤的潜在生物毒性。结果表明，蚯蚓后肠积累了高浓度的砷，导致肠上皮、黄色组织和体腔组织受损。此外，砷污染土壤导致蚯蚓体内甜菜碱水平显著升高，二甲基甘氨酸和肌醇水平降低，这表明蚯蚓的渗透调节代谢可能受到了干扰。天冬酰胺和二甲基甘氨酸水平的显著变化被认为是砷污染土壤的潜在生物标志物。可溶性钙结合蛋白和肌动蛋白结合蛋白的上调、钠/钾转运ATP酶的下调，以及通过基因本体富集分析确定的蛋白质变化，证实蚯蚓遭受了渗透胁迫。此外，甘氨酸-tRNA连接酶活性的显著变化和体腔组织损伤表明，砷的积累可能会干扰蚯蚓的免疫系统。这项工作为蚯蚓体内低水平砷污染土壤生态系统的蛋白质组学和代谢毒性提供了新的见解，扩展了我们对双组学的认识，并突出了毒性的潜在机制。
