College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China.
Qingdao Special Servicemen Recuperation Center of PLA Navy, Qingdao, 266071, China.
BMC Vet Res. 2024 May 8;20(1):182. doi: 10.1186/s12917-024-04034-7.
Porcine cysticercosis, a serious zoonotic parasitic disease, is caused by the larvae of Taenia solium and has been acknowledged by the World Organization for Animal Health. The current detection methods of Cysticercus cellulosae cannot meet the needs of large-scale and rapid detection in the field. We hypothesized that the immunofluorescence chromatography test strip (ICS) for detecting Cysticercus cellulosae, according to optimization of a series of reaction systems was conducted, and sensitivity, specificity, and stability testing, and was finally compared with ELISA. This method utilizes Eu-labeled time-resolved fluorescent microspheres (TRFM) coupled with TSOL18 antigen to detect TSOL18 antibodies in infected pig sera.
ICS and autopsy have highly consistent diagnostic results (n = 133), as determined by Cohen's κ analysis (κ = 0.925). And the results showed that the proposed ICS are high sensitivity (0.9459) with specificity (0.9792). The ICS was unable to detect positive samples of other parasites. It can be stored for at least six months at 4℃.
In summary, we established a TRFM-ICS method with higher sensitivity and specificity than indirect ELISA. Results obtained from serum samples can be read within 10 min, indicating a rapid, user-friendly test suitable for large-scale field detection.
猪囊尾蚴病是一种严重的人畜共患寄生虫病，由猪带绦虫幼虫引起，已被世界动物卫生组织（OIE）承认。目前针对囊尾蚴的检测方法不能满足现场大规模和快速检测的需求。我们假设，根据一系列反应体系的优化，检测囊尾蚴的免疫荧光层析检测试纸条（ICS），并进行了敏感性、特异性和稳定性测试，最后与 ELISA 进行了比较。该方法利用 Eu 标记的时间分辨荧光微球（TRFM）与 TSOL18 抗原结合，检测感染猪血清中的 TSOL18 抗体。
ICS 和剖检具有高度一致的诊断结果（n=133），通过 Cohen's κ 分析（κ=0.925）确定。结果表明，所提出的 ICS 具有较高的灵敏度（0.9459）和特异性（0.9792）。ICS 无法检测其他寄生虫的阳性样本。它可以在 4℃下至少储存 6 个月。
总之，我们建立了一种比间接 ELISA 具有更高灵敏度和特异性的 TRFM-ICS 方法。从血清样本中获得的结果可在 10 分钟内读取，表明这是一种快速、用户友好的适合大规模现场检测的测试方法。
