China-Australian Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang A&F University, Lin'an 311300, China.
Zhejiang University Institute of Preventive Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 310058, China.
J Zhejiang Univ Sci B. 2018;19(11):871-883. doi: 10.1631/jzus.B1800085.
A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
建立了一种一步双流免疫层析分析（DICGA）方法，基于竞争模式，用于同时定量检测玉米、小麦和饲料样品中的赭曲霉毒素 A（OTA）和玉米赤霉烯酮（ZEN）。OTA 的检测限为 0.32ng/ml，检测范围为 0.53-12.16ng/ml，而 ZEN 的检测限为 0.58ng/ml，检测范围为 1.06-39.72ng/ml。在玉米、小麦和饲料样品中的回收率在 77.3%至 106.3%之间，变异系数低于 15%。使用 DICGA 和液相色谱-串联质谱（LC-MS/MS）两种方法对自然污染的玉米、小麦和饲料样品进行了分析，并通过回归分析评估了两种方法之间的相关性。DICGA 方法在食品安全控制中对 OTA 和 ZEN 的简单、快速、灵敏和经济有效的定量检测具有很大的潜力。
