Graduate Program in Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455
Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455.
J Neurosci. 2020 Sep 2;40(36):6910-6926. doi: 10.1523/JNEUROSCI.0763-20.2020. Epub 2020 Aug 7.
Here we examine what effects acute manipulation of the cerebellum, a canonically motor structure, can have on the hippocampus, a canonically cognitive structure. In male and female mice, acute perturbation of the cerebellar vermis (lobule 4/5) or simplex produced reliable and specific effects in hippocampal function at cellular, population, and behavioral levels, including evoked local field potentials, increased hippocampal cFos expression, and altered CA1 calcium event rate, amplitudes, and correlated activity. We additionally noted a selective deficit on an object location memory task, which requires objection-location pairing. We therefore combined cerebellar optogenetic stimulation and CA1 calcium imaging with an object-exploration task, and found that cerebellar stimulation reduced the representation of place fields near objects, and prevented a shift in representation to the novel location when an object was moved. Together, these results clearly demonstrate that acute modulation of the cerebellum alters hippocampal function, and further illustrates that the cerebellum can influence cognitive domains. The cerebellum, a canonically motor-related structure, is being increasingly recognized for its influence on nonmotor functions and structures. The hippocampus is a brain region critical for cognitive functions, such as episodic memory and spatial navigation. To investigate how modulation of the cerebellum may impact the hippocampus, we stimulated two sites of the cerebellar cortex and examined hippocampal function at multiple levels. We found that cerebellar stimulation strongly modulates hippocampal activity, disrupts spatial memory, and alters object-location processing. Therefore, a canonically cognitive brain area, the hippocampus, is sensitive to cerebellar modulation.
在这里，我们研究了急性操纵小脑（经典的运动结构）对海马体（经典的认知结构）的影响。在雄性和雌性小鼠中，急性干扰小脑蚓部（叶 4/5 或 simplex）会在细胞、群体和行为水平上对海马体功能产生可靠且特定的影响，包括诱发局部场电位、增加海马体 cFos 表达以及改变 CA1 钙事件率、幅度和相关活动。我们还注意到在物体位置记忆任务上存在选择性缺陷，该任务需要物体-位置配对。因此，我们将小脑光遗传学刺激和 CA1 钙成像与物体探索任务相结合，发现小脑刺激会减少物体附近位置场的表示，并阻止在物体移动时表示转移到新位置。总之，这些结果清楚地表明，急性小脑调制会改变海马体功能，并进一步表明小脑可以影响认知领域。小脑作为经典的运动相关结构，其对非运动功能和结构的影响正日益受到重视。海马体是大脑中对认知功能（如情景记忆和空间导航）至关重要的区域。为了研究小脑调制如何影响海马体，我们刺激了小脑皮质的两个部位，并在多个层面上检查了海马体的功能。我们发现，小脑刺激强烈调节海马体活动，破坏空间记忆，并改变物体位置处理。因此，一个经典的认知脑区，即海马体，对小脑调制敏感。
