不同草药和合成溶液对牙胶消毒效果的比较评估：一项研究。
Comparative evaluation of various herbal and synthetic solutions on disinfection of guttapercha: An study.
作者信息
Sailaja Pachalla M, Ahmed Sohel, Devi Komera S, Shiva Sandeep
机构信息
Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.
出版信息
Indian J Dent Res. 2020 May-Jun;31(3):376-381. doi: 10.4103/ijdr.IJDR_556_17.
BACKGROUND
Although gutta-percha cones are usually supplied in aseptic packages, once opened and used, they may be contaminated. Chair side disinfection of these cones is important and critical for success of endodontic therapy. Gutta-percha being heat labile, chemical disinfection is the only viable alternative for the sterilization of these cones.
AIM
The aim of the study was to evaluate an effective method of disinfection of gutta-percha cones using various herbal and chemical solutions after intentional contamination with Staphylococcus aureus and Enterococcus faecalis.
MATERIALS AND METHODS
150 gutta-percha cones were selected for the study. Based on the contaminants used, 140 gutta-percha cones were divided into 2 groups: Group A and Group B with 70 cones in each group and the remaining 10 cones (Group VIII) which were uncontaminated served as negative control. The cones from Group A were contaminated with Staphylococcus aureus (S. aureus) and Group B were contaminated with Enterococcus faecalis (E. faecalis). The contaminated cones from both groups were subdivided into 7 groups with 10 cones in each group. Group I cones were disinfected with Q Mix 2 in 1, Group II with 3% sodium hypochlorite, Group III with chitosan, Group IV with aloe vera juice, Group V with amla juice, and Group VI with pancha tulsi. Group VII consisted of gutta-percha cones without any disinfection in both Group A and Group B which served as positive control. The disinfection time was 1 min. All the cones were then incubated in thioglycollate media for 7 days. The thioglycollate media was subcultured and colony forming units were counted. The data were analyzed by one-way ANOVA and post-hoc Tukey tests using SPSS 17.0 software.
RESULTS
There was a statistically significant difference present in the mean colony forming units among all the groups (P < 0.05).
CONCLUSION
Pancha Tulsi and Q Mix 2 in 1 possessed superior antibacterial activity when compared to other experimental solutions.
背景
尽管牙胶尖通常以无菌包装供应，但一旦打开并使用，它们可能会被污染。在椅旁对这些牙胶尖进行消毒对于根管治疗的成功至关重要。由于牙胶对热不稳定，化学消毒是对这些牙胶尖进行灭菌的唯一可行选择。
目的
本研究的目的是评估在故意被金黄色葡萄球菌和粪肠球菌污染后，使用各种草药和化学溶液对牙胶尖进行有效消毒的方法。
材料与方法
选择150个牙胶尖用于本研究。根据所使用的污染物，将140个牙胶尖分为2组：A组和B组，每组70个牙胶尖，其余10个未受污染的牙胶尖（第八组）作为阴性对照。A组的牙胶尖被金黄色葡萄球菌污染，B组的牙胶尖被粪肠球菌污染。两组受污染的牙胶尖再细分为7组，每组10个牙胶尖。第一组牙胶尖用Q Mix 2 in 1消毒，第二组用3%次氯酸钠消毒，第三组用壳聚糖消毒，第四组用芦荟汁消毒，第五组用印度醋栗汁消毒，第六组用五味草消毒。第七组由A组和B组中未进行任何消毒的牙胶尖组成，作为阳性对照。消毒时间为1分钟。然后将所有牙胶尖在硫乙醇酸盐培养基中孵育7天。对硫乙醇酸盐培养基进行传代培养并计数菌落形成单位。使用SPSS 17.0软件通过单因素方差分析和事后Tukey检验对数据进行分析。
结果
所有组之间的平均菌落形成单位存在统计学上的显著差异（P < 0.05）。
结论
与其他实验溶液相比，五味草和Q Mix 2 in 1具有更强的抗菌活性。
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