Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Methods Mol Biol. 2021;2187:207-213. doi: 10.1007/978-1-0716-0814-2_11.
Ceramide can be generated on cell surfaces by the activity of the acid sphingomyelinase. The unique biophysical properties of ceramide result in the self-formation of small ceramide-enriched membrane domains that spontaneously fuse to large ceramide-enriched membrane macrodomains. The present chapter describes how these domains can be labeled and thereby visualized in cells. Further, the chapter provides protocols how ceramide and sphingosine can be quantified on the surface of cells and organs.
神经酰胺可通过酸性鞘磷脂酶的活性在细胞表面生成。神经酰胺独特的物理化学性质导致其自组装成富含神经酰胺的小膜域，这些小膜域自发融合形成富含神经酰胺的大膜域。本章描述了如何对这些域进行标记并在细胞中可视化。此外，本章还提供了在细胞和器官表面定量检测神经酰胺和鞘氨醇的方案。
