超分辨率显微镜观察神经鞘脂

Superresolution Microscopy of Sphingolipids.

机构信息

Department of Biotechnology and Biophysics, Biocenter, University of Wuerzburg, Wuerzburg, Germany.

出版信息

Methods Mol Biol. 2021;2187:303-311. doi: 10.1007/978-1-0716-0814-2_17.

Abstract

This chapter provides a step-by-step protocol to label and visualize sphingolipids by superresolution microscopy with a special focus on single-molecule localization microscopy by dSTORM. We provide information on custom fluorophore conjugation to raft-associated toxins and antibodies, and a labeling protocol for appropriate sample treatment.

摘要

本章提供了一个通过超分辨率显微镜对神经酰胺进行标记和可视化的分步协议，特别关注 dSTORM 的单分子定位显微镜。我们提供了有关定制荧光染料偶联到筏相关毒素和抗体的信息，以及适当的样品处理的标记协议。