• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌F质粒自主控制机制：repE基因产物的纯化与特性分析

Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.

作者信息

Masson L, Ray D S

机构信息

Department of Biology, University of California, Los Angeles 90024.

出版信息

Nucleic Acids Res. 1988 Jan 25;16(2):413-24. doi: 10.1093/nar/16.2.413.

DOI:10.1093/nar/16.2.413
PMID:3277161
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334669/
Abstract

E protein, the 29 Kd repE gene product, is essential for the replication of the Escherichia coli F plasmid. The repE gene has been cloned and expressed in an inducible ATG-fusion vector, and the protein product has been purified to homogeneity. The purified E protein is present as a dimer in solution and binds specifically to both the 19-bp direct repeats (incB) found within the mini-F origin of replication ori2 and the repE operator DNA. Examination of the amino acid sequence of E protein revealed a consensus sequence for DNA binding.

摘要

E蛋白是29千道尔顿的repE基因产物，对大肠杆菌F质粒的复制至关重要。repE基因已被克隆并在可诱导的ATG融合载体中表达，其蛋白质产物已被纯化至同质。纯化后的E蛋白在溶液中以二聚体形式存在，并特异性结合于复制起点ori2的mini-F中发现的19碱基对直接重复序列（incB）和repE操纵子DNA。对E蛋白氨基酸序列的研究揭示了一个DNA结合共有序列。

相似文献

1
Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.大肠杆菌F质粒自主控制机制：repE基因产物的纯化与特性分析
Nucleic Acids Res. 1988 Jan 25;16(2):413-24. doi: 10.1093/nar/16.2.413.
2
Mechanism of autonomous control of the Escherichia coli F plasmid: different complexes of the initiator/repressor protein are bound to its operator and to an F plasmid replication origin.大肠杆菌F质粒自主控制机制：起始/阻遏蛋白的不同复合物与它的操纵基因及F质粒复制起点结合。
Nucleic Acids Res. 1986 Jul 25;14(14):5693-711. doi: 10.1093/nar/14.14.5693.
3
Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid.用于质粒自主复制控制的微小F质粒编码E蛋白的纯化及特性
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4109-13. doi: 10.1073/pnas.83.12.4109.
4
Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).微小F质粒的复制起始蛋白RepE：单体（起始蛋白）与二聚体（自体阻遏物）之间的功能分化
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3839-43. doi: 10.1073/pnas.91.9.3839.
5
Regulation of DNA replication by iterons: an interaction between the ori2 and incC regions mediated by RepE-bound iterons inhibits DNA replication of mini-F plasmid in Escherichia coli.迭代子对DNA复制的调控：由结合RepE的迭代子介导的ori2和incC区域之间的相互作用抑制了大肠杆菌中mini-F质粒的DNA复制。
EMBO J. 1999 Jul 1;18(13):3856-67. doi: 10.1093/emboj/18.13.3856.
6
DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.微小F质粒RepE起始蛋白的DNA结合结构域：羧基末端区域的作用
J Bacteriol. 1995 Apr;177(8):1994-2001. doi: 10.1128/jb.177.8.1994-2001.1995.
7
The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli.通过大肠杆菌的微小F起始蛋白（RepE）以及HU和DnaA的联合作用实现微小F原点的局部解链。
Mol Gen Genet. 1996 Nov 27;253(1-2):42-9. doi: 10.1007/s004380050294.
8
The central region of RepE initiator protein of mini-F plasmid plays a crucial role in dimerization required for negative replication control.微小F质粒的RepE起始蛋白的中央区域在负向复制控制所需的二聚化过程中起着关键作用。
J Mol Biol. 1997 Nov 21;274(1):27-38. doi: 10.1006/jmbi.1997.1373.
9
Mini-F plasmid mutants able to replicate in Escherichia coli deficient in the DnaJ heat shock protein.能够在缺乏DnaJ热休克蛋白的大肠杆菌中复制的微小F质粒突变体。
J Bacteriol. 1992 Sep;174(17):5597-603. doi: 10.1128/jb.174.17.5597-5603.1992.
10
Host control of plasmid replication: requirement for the sigma factor sigma 32 in transcription of mini-F replication initiator gene.宿主对质粒复制的控制：小F复制起始基因转录中对σ因子σ32的需求。
Proc Natl Acad Sci U S A. 1987 Dec;84(24):8849-53. doi: 10.1073/pnas.84.24.8849.

引用本文的文献

1
Spread and Persistence of Virulence and Antibiotic Resistance Genes: A Ride on the F Plasmid Conjugation Module.毒力基因与抗生素抗性基因的传播及持久性：搭乘F质粒接合模块
EcoSal Plus. 2018 Jul;8(1). doi: 10.1128/ecosalplus.ESP-0003-2018.
2
Predicting plasmid promiscuity based on genomic signature.基于基因组特征预测质粒易位。
J Bacteriol. 2010 Nov;192(22):6045-55. doi: 10.1128/JB.00277-10. Epub 2010 Sep 17.
3
Expression and purification of F-plasmid RepE and preliminary X-ray crystallographic study of its complex with operator DNA.

本文引用的文献

1
Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.通过对切割后的miniF片段进行克隆分析来检测和定位六种由miniF编码的蛋白质。
Mol Gen Genet. 1982;186(2):193-203. doi: 10.1007/BF00331850.
2
Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.在微小F质粒复制和不相容性所必需的区域中的九个独特重复序列。
Gene. 1981 Nov;15(2-3):257-71. doi: 10.1016/0378-1119(81)90135-9.
3
Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment.
F 质粒 RepE 的表达与纯化及其与操纵子 DNA 复合物的初步 X 射线晶体学研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1;63(Pt 4):346-9. doi: 10.1107/S1744309107012894. Epub 2007 Mar 30.
4
Specific binding of the replication protein of plasmid pPS10 to direct and inverted repeats is mediated by an HTH motif.
Nucleic Acids Res. 1995 Dec 25;23(24):5048-54. doi: 10.1093/nar/23.24.5048.
5
Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).微小F质粒的复制起始蛋白RepE：单体（起始蛋白）与二聚体（自体阻遏物）之间的功能分化
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3839-43. doi: 10.1073/pnas.91.9.3839.
6
Characterization of the basic replicon of pCM1, a narrow-host-range plasmid from the moderate halophile Chromohalobacter marismortui.嗜盐嗜碱菌死海嗜盐杆菌来源的窄宿主范围质粒pCM1基本复制子的特性分析
J Bacteriol. 1995 Jun;177(12):3443-50. doi: 10.1128/jb.177.12.3443-3450.1995.
7
DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.微小F质粒RepE起始蛋白的DNA结合结构域：羧基末端区域的作用
J Bacteriol. 1995 Apr;177(8):1994-2001. doi: 10.1128/jb.177.8.1994-2001.1995.
8
Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number.广宿主范围质粒RK2的复制：必需的TrfA复制蛋白细胞内浓度的直接测定及其对质粒拷贝数的影响。
J Bacteriol. 1990 Jul;172(7):3849-58. doi: 10.1128/jb.172.7.3849-3858.1990.
9
Site-specific proteolysis of mini-F plasmid replication protein RepE destroys initiator function and generates an incompatibility substance.微小F质粒复制蛋白RepE的位点特异性蛋白水解作用会破坏引发功能并产生一种不相容物质。
J Bacteriol. 1992 May;174(9):3004-10. doi: 10.1128/jb.174.9.3004-3010.1992.
F质粒最小复制区域分离为复制起始片段和反式作用片段。
Mol Gen Genet. 1982;186(3):372-7. doi: 10.1007/BF00729456.
4
An amber replication mutant of F plasmid mapped in the minimal replication region.F质粒的一个琥珀复制突变体定位在最小复制区域。
Mol Gen Genet. 1983;191(2):231-7. doi: 10.1007/BF00334819.
5
Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control.F 小基因组的九个重复序列在 F 特异性不相容表型表达和拷贝数控制中的作用。
J Bacteriol. 1983 Jul;155(1):337-44. doi: 10.1128/jb.155.1.337-344.1983.
6
Genetic evidence that control of F replication is negative.F因子复制受负调控的遗传学证据。
Mol Gen Genet. 1982;187(2):218-24. doi: 10.1007/BF00331120.
7
An Mr 29000 protein is essential for mini-F maintenance in E. coli.一种分子量为29000的蛋白质对大肠杆菌中微型F质粒的维持至关重要。
Gene. 1982 Sep;19(2):173-8. doi: 10.1016/0378-1119(82)90003-8.
8
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳研究乳糖阻遏物-操纵基因相互作用的平衡与动力学
Nucleic Acids Res. 1981 Dec 11;9(23):6505-25. doi: 10.1093/nar/9.23.6505.
9
Protein-DNA recognition.蛋白质-脱氧核糖核酸识别
Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453.
10
Regulation of plasmid replication.质粒复制的调控
Microbiol Rev. 1984 Mar;48(1):1-23. doi: 10.1128/mr.48.1.1-23.1984.