Department of Food Science, Cornell University, Ithaca, NY 14850.
Department of Food Science, Cornell University, Ithaca, NY 14850; School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850.
J Dairy Sci. 2020 Oct;103(10):8796-8807. doi: 10.3168/jds.2020-18697. Epub 2020 Aug 6.
A new strategy to concentrate phospholipids from buttermilk powder was developed using a food-grade green method based on ethanol-modified supercritical carbon dioxide (SC-CO) extraction. The effects of extraction conditions, namely temperature (50 and 60°C), pressure (30 and 40 MPa), and ethanol concentration (10, 15, and 20%, wt/wt), on the total lipid yield and phospholipid content were investigated. The ethanol concentration had a more significant effect on the total lipid yield and phospholipid content than the temperature and pressure within the ranges studied. The highest phospholipid recovery was achieved at 60°C, 30 MPa, and 15% (wt/wt) ethanol with a total lipid yield of 6.3% (wt/wt), of which 49% (wt/wt) were phospholipids composed of dihydrosphingomyelin (5%), sphingomyelin (24%), phosphatidylethanolamine (22%), phosphatidylserine (2%), phosphatidylinositol (3%), and phosphatidylcholine (44%). The triacylglycerol compositions of extracts obtained by Folch and ethanol-modified SC-CO extractions were similar. A sequential pure SC-CO and ethanol-modified SC-CO extraction was carried out to separate nonpolar lipids in the first fraction, thereby concentrating phospholipids in the second fraction. This sequential extraction produced a highly concentrated phospholipid extract (76%, wt/wt). To the best of our knowledge, this is the highest phospholipid concentration reported from buttermilk powder. Thus, this phospholipid-rich extract can be used in the development of functional foods as a food-grade emulsifier with potential health-promoting effects.
开发了一种从乳清蛋白粉中浓缩磷脂的新策略，该策略使用基于乙醇改性超临界二氧化碳（SC-CO2）提取的食品级绿色方法。考察了提取条件（温度为 50 和 60°C，压力为 30 和 40 MPa，乙醇浓度为 10、15 和 20%，wt/wt）对总脂质得率和磷脂含量的影响。在研究范围内，乙醇浓度对总脂质得率和磷脂含量的影响比对温度和压力的影响更为显著。在 60°C、30 MPa 和 15%（wt/wt）乙醇下，磷脂回收率最高，总脂质得率为 6.3%（wt/wt），其中 49%（wt/wt）为二氢神经鞘氨醇（5%）、神经鞘磷脂（24%）、磷脂酰乙醇胺（22%）、磷脂酰丝氨酸（2%）、磷脂酰肌醇（3%）和磷脂酰胆碱（44%）组成的磷脂。Folch 和乙醇改性 SC-CO2 提取得到的提取物中的三酰基甘油组成相似。进行了顺序纯 SC-CO2 和乙醇改性 SC-CO2 提取，以在第一馏分中分离非极性脂质，从而在第二馏分中浓缩磷脂。这种顺序提取产生了高度浓缩的磷脂提取物（76%，wt/wt）。据我们所知，这是从乳清蛋白粉中报道的最高磷脂浓度。因此，这种富含磷脂的提取物可用作功能性食品中的食品级乳化剂，具有潜在的促进健康的作用。
