Piemjai Morakot, Chantarawej Pareewan, Nakabayashi Nobuo
Department of Prosthodontics, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Int J Dent. 2020 Jul 22;2020:5859835. doi: 10.1155/2020/5859835. eCollection 2020.
Secondary caries originate from a leakage pathway where oral acids can penetrate faster and demineralize the tooth substrate deeper which can be visualized by dye penetration. The ability to prevent secondary caries by contemporary adhesive systems was evaluated in this study. Dye penetration distance through leakage and into the tooth substrate adjacent to Class V restorations after artificial caries exposure was compared.
Previously frozen extracted human molars were used to prepare the Class V cavities at the CEJ on axial surfaces. All cavities were restored with either the resin-composite or amalgam with or without resin adhesives: dry bonding: Super-Bond D-Liner II Plus; moist bonding: All-Bond 2; and self-etch bonding: AQ Bond and Clearfil Protect Bond. Two subgroups of Super-Bond D-Liner II Plus were immersed for 14 days at 37°C either in artificial saliva (negative control) or the artificial caries solution. The other groups were soaked in the artificial caries solution. The distance of dye penetration into the adjacent enamel, cementum/dentin, and tooth-resin interfaces was measured after immersion in 0.5% basic fuchsin dye for 24 h. The tooth-resin interfacial layer was investigated using SEM.
No dye penetration into the tooth-resin interface was found in Super-Bond D-Liner II Plus and AQ Bond groups which demonstrated a constant hybrid layer after a chemical challenge. The leakage distance at the cementum/dentin-resin interface of All-Bond 2, Clearfil Protect Bond, and non-adhesive amalgam (positive control) groups was significantly higher than the distance of dye penetration into the adjacent demineralized root surface ( < 0.05).
Caries associated with either amalgam or resin-composite restorations can be prevented using resin adhesives which can penetrate into the intact tooth substrate to form a stable hybrid layer. With caries-free restorations, tooth vitality may be conserved lifelong.
继发龋源于渗漏通道，口腔酸可更快地穿透该通道并使牙体组织更深层脱矿，这可通过染料渗透来观察。本研究评估了当代粘接系统预防继发龋的能力。比较了人工龋暴露后染料通过渗漏进入Ⅴ类洞修复体邻近牙体组织的渗透距离。
使用先前冷冻提取的人类磨牙在轴向表面的牙骨质牙本质界处制备Ⅴ类洞。所有窝洞均用树脂复合材料或汞合金修复，使用或不使用树脂粘接剂：干式粘接：Super-Bond D-Liner II Plus；湿式粘接：All-Bond 2；自酸蚀粘接：AQ Bond和Clearfil Protect Bond。将Super-Bond D-Liner II Plus的两个亚组在37℃下于人工唾液（阴性对照）或人工龋溶液中浸泡14天。其他组浸泡在人工龋溶液中。在0.5%碱性品红染料中浸泡24小时后，测量染料渗入邻近釉质、牙骨质/牙本质和牙-树脂界面的距离。使用扫描电子显微镜研究牙-树脂界面层。
在Super-Bond D-Liner II Plus和AQ Bond组中未发现染料渗入牙-树脂界面，在化学刺激后显示出恒定的混合层。All-Bond 2、Clearfil Protect Bond和非粘接性汞合金（阳性对照）组在牙骨质/牙本质-树脂界面的渗漏距离显著高于染料渗入邻近脱矿根面的距离（P<0.05）。
使用能够渗入完整牙体组织以形成稳定混合层的树脂粘接剂，可以预防与汞合金或树脂复合材料修复体相关的龋齿。对于无龋修复体，牙齿活力可终身保存。
