Chetwynd Andrew J, Wheeler Korin E, Lynch Iseult
School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, UK.
Department of Chemistry and Biochemistry, Santa Clara University, 500 El Camino Real, Santa Clara, CA 95053, United States.
Nano Today. 2019 Oct;28. doi: 10.1016/j.nantod.2019.06.004. Epub 2019 Aug 17.
Characterisation of the adsorption of biomolecules, or a biocorona, on nanomaterials has proliferated in the past 10 years, as protein corona studies provide molecular level insight into mechanisms of cellular recognition, uptake, and toxicity of nanomaterials. At the crossroads of two rapidly evolving orthogonal fields, nanoscience and proteomics, the interdisciplinarity of protein corona studies creates challenges for experimental design and reporting. Here we propose a flexible checklist for experimental design and reporting guidelines to outline Minimum Information about Nanomaterial Biocorona Experiments (MINBE). The checklist for experimental design, compiled after review of reporting within the protein corona literature, provides researchers with prompts to ensure best practice experimental approaches for each stage of the workflow, collated from the nanoscience, proteomics, and bioinformatics fields. Reporting guidelines are also assembled from established sources, integrated to span the entire workflow and extended and modified to aid interdisciplinary researchers in the most challenging stages of the workflow. Where appropriate, de novo guidelines to address areas specific to protein corona studies, including exposure conditions and isolation of adsorbed proteins, were written. The MINBE guidelines provide protein corona researchers with a conduit between materials science techniques and proteomics. Implementation of these guidelines is anticipated to catalyse enhanced quality, impact, and extent of data mining and computational modelling of protein corona composition and its role in nanosafety and nanomedicine. Furthermore, high quality experimental design and reporting in the bio-nanosciences will enhance the next phase of targeted nanomedicines and sustainable nanotechnologies.
在过去十年中，生物分子或生物冠层在纳米材料上的吸附表征研究大量涌现，因为蛋白质冠层研究能在分子水平上深入了解纳米材料的细胞识别、摄取和毒性机制。处于纳米科学和蛋白质组学这两个快速发展的正交领域的交叉点，蛋白质冠层研究的跨学科性给实验设计和报告带来了挑战。在此，我们提出一份灵活的实验设计清单和报告指南，以概述纳米材料生物冠层实验的最小信息（MINBE）。该实验设计清单是在回顾蛋白质冠层文献中的报告后编制的，为研究人员提供了提示，以确保在工作流程的每个阶段都采用最佳实践实验方法，这些方法是从纳米科学、蛋白质组学和生物信息学领域整理而来的。报告指南也取自既定来源，整合后涵盖整个工作流程，并进行了扩展和修改，以帮助跨学科研究人员应对工作流程中最具挑战性的阶段。在适当的情况下，还编写了针对蛋白质冠层研究特定领域的全新指南，包括暴露条件和吸附蛋白质的分离。MINBE指南为蛋白质冠层研究人员提供了材料科学技术与蛋白质组学之间的桥梁。预计实施这些指南将促进蛋白质冠层组成的数据挖掘和计算建模的质量、影响力及范围的提升，以及其在纳米安全和纳米医学中的作用。此外，生物纳米科学中的高质量实验设计和报告将推动靶向纳米药物和可持续纳米技术的下一阶段发展。
