Wang Zaiqiang, Zang Yu, Gao Yanjun, Han Luyao, Lin Hongwei, Gao Yongheng, Chen Min, Liu Yurou, Zhang Qian, Fu Enqing
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Air Force Medical University Xi'an 710038, P. R. China.
Am J Transl Res. 2020 Jul 15;12(7):4009-4016. eCollection 2020.
The clinical application of the loop-mediated isothermal amplification (LAMP) assay has been problematic because of conflicting results obtained from the LAMP assay and bacterial culture. In order to eliminate the interference of oral microorganisms and more accurately evaluate the diagnostic performance of the LAMP assay, we utilized bronchoalveolar lavage fluid (BALF) as a sample to test whether the LAMP assay and bacteria culture yielded similar results. A total of 1092 BALF samples from patients with suspected lower respiratory tract infections were collected. For each sample, parallel studies using both bacterial culture and the LAMP assay were carried out. We were the first to utilize BALF as a sample to study the consistency between the LAMP assay and bacterial culture results. The present study demonstrated that the positive rate from the LAMP assay was higher than that from bacterial culture, and the two methods had a better consistency than previously reported.
由于环介导等温扩增（LAMP）检测法与细菌培养结果存在矛盾，其临床应用一直存在问题。为消除口腔微生物的干扰并更准确地评估LAMP检测法的诊断性能，我们采用支气管肺泡灌洗液（BALF）作为样本，以测试LAMP检测法和细菌培养是否产生相似结果。共收集了1092例疑似下呼吸道感染患者的BALF样本。对每个样本同时进行细菌培养和LAMP检测法的平行研究。我们首次利用BALF作为样本研究LAMP检测法与细菌培养结果之间的一致性。本研究表明，LAMP检测法的阳性率高于细菌培养，且两种方法的一致性比先前报道的更好。
