Minhas Amritpreet Kaur, Gaur Suchitra, Adholeya Alok
TERI Deakin Nanobiotechnology Centre, The Energy and Resources Institute, New Delhi, India.
Heliyon. 2023 Jan 13;9(2):e12801. doi: 10.1016/j.heliyon.2023.e12801. eCollection 2023 Feb.
Microalgal biomass has the ability to store huge amount of triacylglycerides as fatty ester methyl esters (FAME) and carotenoids which has made algae as potential candidate for biorefinery approach. Essential fatty acid such as palmitic acid, stearic acid, arachidonic acid and eicospentanoic acid have been produced which are known for their various applications. The present study was aimed to evaluate the influence of different light intensities (120 and 250 μE/m/s) and photoperiod (16:8h and 13:11h light/dark cycle) on the production of lipid, biomass and lutein. and was grown for 23 days in F/2, sea salt media (SSM, Distilled water (DW) and SSM (natural seawater media,NSW) under two different light intensities and photoperiod regimes at 25 ᵒC. SSM (NSW) showed maximum accumulation of lipid in (34.56 mg/L/d). SSM (DW)- biomass showed 1.5 times higher lutein productivity of 0.253 mg/L/d under 13:11h light/dark cycle at 250 μE/m/s compared to same medium under 16:8h light/dark cycles at 120 μE/m/s. Where as in , F/2 biomass showed higher lipid and lutein productivity of 15.69 and 0.279 mg/L/d, respectively The laboratory scale cultivation parameters and related media cost showed the suitability of different culture media adaptation to large scale production.
微藻生物质有能力储存大量甘油三酯作为脂肪酸甲酯（FAME）和类胡萝卜素，这使得藻类成为生物精炼方法的潜在候选者。已经生产出了诸如棕榈酸、硬脂酸、花生四烯酸和二十碳五烯酸等必需脂肪酸，它们因其各种应用而闻名。本研究旨在评估不同光照强度（120和250μE/m/s）和光周期（16:8小时和13:11小时光/暗循环）对脂质、生物质和叶黄素产量的影响。 在25℃下，在两种不同光照强度和光周期条件下，于F/2、海盐培养基（SSM，蒸馏水（DW）和SSM（天然海水培养基，NSW）中培养23天。SSM（NSW）显示出 中脂质的最大积累量为（34.56mg/L/d）。与在120μE/m/s的16:8小时光/暗循环下的相同培养基相比，SSM（DW）-生物质在250μE/m/s的13:11小时光/暗循环下叶黄素生产率高出1.5倍，为0.253mg/L/d。而在 中，F/2生物质分别显示出更高的脂质和叶黄素生产率，为15.69和0.279mg/L/d。实验室规模的培养参数和相关培养基成本表明了不同培养基适用于大规模生产。
