Haruna Ishaku L, Hadebe Sibusiso A, Oladosu Oyekunle J, Mahmoud Ghassan, Zhou Huitong, Hickford Jon G H
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand.
Arch Anim Breed. 2020 Jul 22;63(2):241-248. doi: 10.5194/aab-63-241-2020. eCollection 2020.
Leptin is mainly secreted by white adipose tissue in animals. Leptin acts by stimulating or inhibiting the release of a neurotransmitter, which eventually results in a decrease in food/feed intake and an increase in energy expenditure. In this investigation, the polymerase chain reaction (PCR) coupled with single-strand conformation polymorphism (SSCP) analysis was used to reveal nucleotide sequence variations in bovine leptin gene () in 338 cattle of a variety of breeds farmed in New Zealand (NZ) and Nigeria. These included NZ Hereford, Angus, Shorthorn, and crossbred Holstein-Friesian Jersey cattle and the Nigerian Sokoto Gudali, Red Bororo, White Fulani, and crossbred Holstein-Friesian White Fulani cattle. Sequence analysis of three regions of bovine that encompassed selected coding and non-coding regions, revealed a total of 12 nucleotide sequence variations (six in exons and six in introns). Of these, three are reported here for the first time, whereas nine have been previously described. Some of the variations identified were common in both the NZ and Nigerian cattle breeds, while others were peculiar to particular breeds from a specific region. The sharing of common variants across different breeds irrespective of geography may indicate an evolutionary relationship, just as the differences within a breed might be attributable to either selective pressure for specific traits or random genetic drift. The detection of both new and previously documented variations in bovine suggests that the gene is highly variable.
瘦素主要由动物体内的白色脂肪组织分泌。瘦素通过刺激或抑制神经递质的释放来发挥作用，最终导致食物/饲料摄入量减少和能量消耗增加。在本研究中，聚合酶链反应（PCR）结合单链构象多态性（SSCP）分析，用于揭示新西兰（NZ）和尼日利亚养殖的338头不同品种牛的牛瘦素基因（）的核苷酸序列变异。这些品种包括新西兰赫里福德牛、安格斯牛、短角牛以及杂交的荷斯坦-弗里生牛和泽西牛，还有尼日利亚索科托古达利牛、红博罗罗牛、白富拉尼牛以及杂交的荷斯坦-弗里生牛和白富拉尼牛。对牛的三个区域进行序列分析，这些区域涵盖了选定的编码区和非编码区，共发现12个核苷酸序列变异（6个在外显子中，6个在内含子中）。其中，3个变异是首次在此报道，而另外9个变异此前已有描述。所鉴定出的一些变异在新西兰和尼日利亚的牛品种中都很常见，而其他一些变异则特定于来自特定区域的特定品种。无论地理位置如何，不同品种间共有变异的存在可能表明它们之间存在进化关系，正如一个品种内的差异可能归因于对特定性状的选择压力或随机遗传漂变一样。牛基因中既有新发现的变异，也有先前记录的变异，这表明该基因具有高度变异性。
