Baruthio F, Guillard O, Arnaud J, Pierre F, Zawislak R
INRS, Service Epidémiologie, Vandoeuvre, France.
Clin Chem. 1988 Feb;34(2):227-34.
The great diversity of methods for measuring manganese in biological materials (serum, plasma, whole blood, urine, spinal fluid, and hair) reflects the difficulty in measuring extremely small quantities of this element. Detailed examination of these methods demonstrates that the one most used is flameless atomic absorption spectrometry. In this review we report the different instrument settings for wavelength, slit width, protection gases, graphite furnaces, type of background correction, amounts measured, and thermal programs. We give detailed recommendations by various authors for collecting samples. A thorough description of the preliminary steps and the handling of the specimen samples is also included: direct determination with or without dilution, addition of a matrix modifier or determination after ashing, with or without chelation-extraction steps. The preparation of the standards, procedures used, analytical criteria (accuracy, precision, specificity, detection limit, linearity), problems (interferences, matrix effects), and reference values and their physiological variations are also described. We give a consensus of recommendations concerning the choice of a method.
用于测量生物材料（血清、血浆、全血、尿液、脑脊液和毛发）中锰的方法种类繁多，这反映出测量极微量该元素存在困难。对这些方法的详细研究表明，最常用的方法是无火焰原子吸收光谱法。在本综述中，我们报告了波长、狭缝宽度、保护气体、石墨炉、背景校正类型、测量量和热程序等不同的仪器设置。我们给出了不同作者关于采集样本的详细建议。还包括对初步步骤和样本处理的全面描述：直接测定（有无稀释）、添加基体改进剂或灰化后测定（有无螯合萃取步骤）。还描述了标准品的制备、使用的程序、分析标准（准确性、精密度、特异性、检测限、线性）、问题（干扰、基体效应）以及参考值及其生理变化。我们就方法的选择给出了一致的建议。
