Ball P E, Munzer H, Keller H P, Abisch E, Rosenthaler J
Department of Biopharmaceutics, Sandoz Ltd., Basel, Switzerland.
Clin Chem. 1988 Feb;34(2):257-60.
A specific radioimmunoassay involving a mouse monoclonal antibody to cyclosporine has been developed for monitoring the parent drug in blood. Pretreatment with methanol removes cyclosporine from the erythrocytes. The limit of detection is about 12 micrograms/L, sample volume is 50 microL of blood, and within- and between-assay CVs are less than 7%. Assay results correlated well with those obtained by "high-performance" liquid chromatography (HPLC) for liver (n = 42), for heart (n = 64), for bone-marrow (n = 36), and for kidney (n = 140). For blood specimens obtained from patients treated with cyclosporine postoperatively for as long as 65 months, the mean RIA/HPLC ratio in all with transplant indications was close to 1. Therefore, the specific radioimmunoassay apparently can be used instead of HPLC to measure the parent drug in blood.
已开发出一种特定的放射免疫分析法，该方法使用一种针对环孢素的小鼠单克隆抗体来监测血液中的母体药物。用甲醇预处理可从红细胞中去除环孢素。检测限约为12微克/升，样本体积为50微升血液，批内和批间变异系数均小于7%。该分析结果与通过“高效”液相色谱法（HPLC）在肝脏（n = 42）、心脏（n = 64）、骨髓（n = 36）和肾脏（n = 140）中获得的结果高度相关。对于术后接受环孢素治疗长达65个月的患者所采集的血液样本，所有有移植指征患者的放射免疫分析/高效液相色谱法平均比值接近1。因此，这种特定的放射免疫分析法显然可以替代高效液相色谱法来测定血液中的母体药物。
