Department of Tumor Molecular Markers, N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation, 24 Kashirskoe Shosse, Moscow, Russia.
Oncological Department of Surgery no. 2, N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation, 23 Kashirskoe Shosse, Moscow, Russia.
Biotechniques. 2020 Oct;69(4):257-263. doi: 10.2144/btn-2020-0024. Epub 2020 Aug 11.
A new method of double immunofluorescent staining for flow cytometry has been created to evaluate quantitative expression of mesenchymal protein vimentin only in epithelial cells of a solid tumor that is a mix of different origin cells. vimentin expression is strongly associated with epithelial-mesenchymal transition and therefore is a metastatic potential marker of epithelial tumor cells. In comparison with semiquantitative available methods, the proposed one has several advantages, such as the accurate measurement of the marker's expression, and minimization of spatial and temporal tumor heterogeneity. Clinical validation of the method has revealed inverse correlation between the quantitative index of epithelial-mesenchymal transition level and progression-free survival using Kaplan-Meier curves and the COX proportional hazards ratio in 32 ovarian cancer patients.
创建了一种新的流式细胞术双免疫荧光染色方法，用于评估在由不同来源细胞混合组成的实体瘤上皮细胞中，间充质蛋白波形蛋白的定量表达情况。波形蛋白的表达与上皮-间充质转化密切相关，因此是上皮肿瘤细胞转移潜能的标志物。与现有的半定量方法相比，该方法具有几个优点，如标志物表达的准确测量和肿瘤异质性的时空最小化。该方法的临床验证显示，在 32 名卵巢癌患者中，使用 Kaplan-Meier 曲线和 COX 比例风险比，上皮-间充质转化水平的定量指数与无进展生存期呈负相关。
