百日咳毒素对瑞士3T3细胞中生长因子刺激的肌醇磷酸形成和DNA合成的影响。
Effects of pertussis toxin on growth factor-stimulated inositol phosphate formation and DNA synthesis in Swiss 3T3 cells.
作者信息
Taylor C W, Blakeley D M, Corps A N, Berridge M J, Brown K D
机构信息
AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, Cambridge, U.K.
出版信息
Biochem J. 1988 Feb 1;249(3):917-20. doi: 10.1042/bj2490917.
Abstract
We have compared the effects of pretreatment of Swiss 3T3 cell with pertussis toxin on the stimulation of DNA synthesis and phosphoinositide hydrolysis in response to a wide variety of mitogens. The toxin substantially inhibited the stimulation of DNA synthesis in response to a phorbol ester or various peptide and polypeptide growth factors irrespective of their ability to activate phosphoinositidase C. Production of inositol phosphates in response to platelet-derived growth factor, fibroblast growth factor and prostaglandin F2 alpha were unaffected by the toxin while bombesin- and vasopressin-stimulated formation of inositol phosphates were inhibited by only 27 and 23% respectively. These results argue against a major role for a pertussis toxin-sensitive G protein in coupling any of these mitogen receptors to activation of a phosphoinositidase C. Furthermore, the results suggest that the widespread inhibitory effects of pertussis toxin on mitogen-stimulated DNA synthesis may be unrelated to the toxin's limited actions on phosphoinositide hydrolysis.
摘要
我们比较了用百日咳毒素预处理瑞士3T3细胞对多种有丝分裂原刺激的DNA合成和磷酸肌醇水解的影响。该毒素显著抑制了佛波酯或各种肽及多肽生长因子刺激的DNA合成，无论它们激活磷脂酶C的能力如何。血小板衍生生长因子、成纤维细胞生长因子和前列腺素F2α刺激产生的肌醇磷酸不受该毒素影响，而蛙皮素和血管加压素刺激的肌醇磷酸形成仅分别被抑制27%和23%。这些结果表明，百日咳毒素敏感的G蛋白在将这些有丝分裂原受体中的任何一种与磷脂酶C的激活偶联方面不起主要作用。此外，结果表明百日咳毒素对有丝分裂原刺激的DNA合成的广泛抑制作用可能与该毒素对磷酸肌醇水解的有限作用无关。
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