Department of Geriatric, Tianjin Medical University General Hospital, Tianjin, China.
Department of Orthopedics, Tianjin First Center Hospital Tianjin, China.
J Clin Lab Anal. 2020 Nov;34(11):e23471. doi: 10.1002/jcla.23471. Epub 2020 Aug 11.
Osteoporosis is one of the most closely related diseases associated with the elderly. In recent years, the studies found that gut microbiota can cause osteoporosis. We evaluated the role of Bacillus acidophilus in osteoporosis and its roles in proliferation and differentiation.
We selected 5 healthy people and 10 osteoporosis patients and analyzed their level of 25-hydroxyvitamin D and procollagen type I N-terminal peptide (PINP), the characteristic of gut microbiota. The effect of lactobacillus acidophilus and Lactobacillus rhamnosus supernatant and butanoic acids on proliferation, differentiation, and maturity of osteoblasts MC3T3-E1 and osteoclasts RAW 264.7 cells and the activity of alkaline phosphatase, concentration of osteocalcin, and the expression of RUNX2, RANK, NFATc1, cathepsin K, DC-STAMP, OSCAR, WNT2, and CTNNB1 were measured in the above cell lines.
The diversity of gut microbiota in osteoporosis patients is decreased and imbalanced with lower abundance of lactobacillus and butyric acid bacteria; meanwhile, 25-hydroxyvitamin D and PINP of osteoporosis patient were significantly lower than the normal group. The proliferation, differentiation, and maturity of MC3T3-E1 cells were stimulated; the activity of alkaline phosphatase, concentration of osteocalcin, and the expression of RUNX2, NFATc1, cathepsin K, DC-STAMP, OSCAR, WNT2, and CTNNB1 were improved by supernatant of lactobacillus acidophilus, Lactobacillus rhamnosus and butanoic acids; however, the proliferation, differentiation, maturity, and the expression of RANK, NFATc1, cathepsin K, DC-STAMP, OSCAR, WNT2, and CTNNB1 in RAW 264.7 cells were suppressed.
The lactobacillus acidophilus and Lactobacillus rhamnosus supernatant could stimulate the proliferation, differentiation, and maturation of osteoblasts; the production of butyric acid may be the potential mechanism.
骨质疏松症是与老年人最密切相关的疾病之一。近年来，研究发现肠道微生物群会导致骨质疏松症。我们评估了嗜酸乳杆菌在骨质疏松症中的作用及其在增殖和分化中的作用。
我们选择了 5 名健康人和 10 名骨质疏松症患者，分析了他们 25-羟维生素 D 和前胶原 I N 端肽（PINP）的水平，以及肠道微生物群的特征。嗜酸乳杆菌和鼠李糖乳杆菌上清液和丁酸对成骨细胞 MC3T3-E1 和破骨细胞 RAW 264.7 细胞增殖、分化和成熟的影响，以及碱性磷酸酶活性、骨钙素浓度和 RUNX2、RANK、NFATc1、组织蛋白酶 K、DC-STAMP、OSCAR、WNT2 和 CTNNB1 的表达在上述细胞系中进行了测量。
骨质疏松症患者肠道微生物群的多样性降低且失衡，乳杆菌和丁酸杆菌的丰度降低；同时，骨质疏松症患者的 25-羟维生素 D 和 PINP 明显低于正常组。MC3T3-E1 细胞的增殖、分化和成熟受到刺激；嗜酸乳杆菌、鼠李糖乳杆菌上清液和丁酸可提高碱性磷酸酶活性、骨钙素浓度以及 RUNX2、NFATc1、组织蛋白酶 K、DC-STAMP、OSCAR、WNT2 和 CTNNB1 的表达；然而，RAW 264.7 细胞的增殖、分化、成熟以及 RANK、NFATc1、组织蛋白酶 K、DC-STAMP、OSCAR、WNT2 和 CTNNB1 的表达受到抑制。
嗜酸乳杆菌和鼠李糖乳杆菌上清液可刺激成骨细胞增殖、分化和成熟；丁酸的产生可能是潜在的机制。
