Frazier M W, Mosig G
Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235.
J Bacteriol. 1988 Mar;170(3):1384-8. doi: 10.1128/jb.170.3.1384-1388.1988.
We have analyzed early and late T4 gene expression at the levels of transcription and translation in rpoH+ (sigma 32+) and rpoH mutant cells infected under heat shock conditions. We found, as expected, that Escherichia coli cells must be adapted before infection to high temperature by the heat shock response to allow early T4 transcription, subsequent late gene expression, and progeny production at 42 degrees C. Unexpectedly, we found in addition that when rpoH mutant (sigma 32 mutant) cells were shifted from 30 to 42 degrees C 10 min after infection, late T4 genes were not expressed, even though DNA synthesis appeared to be normal.
我们已经在转录和翻译水平上分析了在热休克条件下感染的rpoH+（σ32+）和rpoH突变体细胞中T4基因的早期和晚期表达。正如预期的那样，我们发现，大肠杆菌细胞在感染前必须通过热休克反应适应高温，以便在42℃时进行早期T4转录、随后的晚期基因表达和子代产生。出乎意料的是，我们还发现，当rpoH突变体（σ32突变体）细胞在感染后10分钟从30℃转移到42℃时，即使DNA合成似乎正常，晚期T4基因也不表达。
