Department of Paraclinical Veterinary Studies, Faculty of Veterinary Science, University of Zimbabwe, Harare, Zimbabwe.
Central Veterinary Laboratory, Division of Veterinary Technical Services, Ministry of Lands, Agriculture, Water and Rural Resettlement, Harare, Zimbabwe.
Trop Anim Health Prod. 2020 Nov;52(6):3323-3330. doi: 10.1007/s11250-020-02364-w. Epub 2020 Aug 11.
Resistance of one host and three host ticks on cattle to amitraz was studied using samples from five diptanks in the Domboshawa Communal Land Area of Zimbabwe. A random tick profile and a questionnaire survey on the tick control practices of the area were also carried out. Engorged Rhipicephalus (Boophilus) decoloratus, Rhipicephalus appendiculatus and Amblyomma hebraeum females were randomly collected from cattle presented for dipping at the 5 diptanks and were allowed to oviposit separately at T: 28 °C and RH: 85-95%. Larvae obtained were tested for resistance against various amitraz concentrations (1-0.0078125%) using the Larval Packet Test (LPT) and were compared with susceptible reference strains of R. (B.) decoloratus (Makuti strain, 2017), R. appendiculatus (Lake Chivero strain, 2015) and A. hebraeum (Lake Mutirikwi strain, 2017). The most abundant tick species were R. (B.) decoloratus (27.2%), Hyalomma rufipes (20.0%), H. truncatum (16.0%), R. appendiculatus (12.0%) and R. evertsi evertsi (11.9%). Amblyomma hebraeum (8.6%) and A. variegatum (1.8%) were the least common in the collection; this suggests that they were not well established in Domboshawa. Low amitraz resistance (RL = I) was detected only in R. (B.) decoloratus at 2 of the 5 diptanks. In the future, decentralised tick control due to inadequate and inconsistent supply of acaricides could introduce a number of factors which could contribute towards resistance development.
在津巴布韦多姆博绍瓦社区土地地区的五个浸浴池中，使用来自五个浸浴池的样本研究了一种宿主和三种宿主蜱对阿密特的抗性。还进行了随机蜱概况和该地区蜱控制实践的问卷调查。从 5 个浸浴池中出现的牛身上随机采集了饱血的 Rhipicephalus（Boophilus）decoloratus、Rhipicephalus appendiculatus 和 Amblyomma hebraeum 雌性，并分别在 T：28°C 和 RH：85-95%下允许产卵。获得的幼虫使用幼虫包测试（LPT）测试对各种阿密特浓度（1-0.0078125%）的抗性，并与易感的 R.（B.）decoloratus（Makuti 株，2017 年）、R. appendiculatus（Lake Chivero 株，2015 年）和 A. hebraeum（Lake Mutirikwi 株，2017 年）的参考敏感株进行比较。最丰富的蜱种是 R.（B.）decoloratus（27.2%）、Hyalomma rufipes（20.0%）、H. truncatum（16.0%）、R. appendiculatus（12.0%）和 R. evertsi evertsi（11.9%）。在收集的蜱中，Amblyomma hebraeum（8.6%）和 A. variegatum（1.8%）最少，这表明它们在多姆博绍瓦尚未很好地建立。仅在 5 个浸浴池中的 2 个中检测到 R.（B.）decoloratus 的低阿密特抗性（RL = I）。未来，由于杀蜱剂供应不足且不一致，分散的蜱控制可能会引入一些因素，这些因素可能导致抗性发展。
