Xu Lingqing, Deng Shufei, Wen Weihong, Tang Yingxian, Chen Linjuan, Li Yuzhen, Zhong Guoquan, Li Jiehua, Ting Wei-Jen, Fu Bishi
Department of Clinical Laboratory, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, Guangdong 511518, P.R. China.
School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 511436, P.R. China.
Exp Ther Med. 2020 Sep;20(3):1943-1952. doi: 10.3892/etm.2020.8911. Epub 2020 Jun 19.
The present study aimed to investigate the association between drug resistance and class I, II and III integrons in (ABA). Multilocus sequence typing (MLST) is a tool used to analyze the homology among house-keeping gene clusters in ABA and ABA prevalence and further provides a theoretical basis for hospitals to control ABA infections. A total of 96 clinical isolates of non-repeating ABA were harvested, including 74 carbapenem-resistant ABA (CRABA) and 22 non-CRABA strains, and used for bacterial identification and drug susceptibility analysis. Variable regions were sequenced and analyzed. Then, 7 pairs of housekeeping genes were amplified and sequenced via MLST and sequence alignment was performed against the Pub MLST database to determine sequence types (STs) strains and construct different genotypic evolutionary diagrams. The detection rate of CRABA class I integrons was 13.51% (10/74); no class II and III integrons were detected. However, class I, II and III integrons were not detected in non-CRABA strains. The variable regions of 9 of 10 class I integrons were amplified and 10 gene cassettes including aacC1, aac1, aadDA1, aadA1a, aacA4, dfrA17, aadA5, aadA1, aadA22 and aadA23 were associated with drug resistance. The 96 ABA strains were divided into 21 STs: 74 CRABA strains containing 9 STs, primarily ST208 and ST1145 and 22 non-CRABA strains containing 18 STs, primarily ST1145. Class I integrons are a critical factor underlying drug resistance in ABA. CRABA and non-CRABA strains differ significantly; the former primarily contained ST208 and ST1145, and the latter contained ST1145. Most STs were concentrated in intensive care units (ICUs) and the department of Neurology, with the patients from the ICUs being the most susceptible to bacterial infection. In the Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, ABA is potentially horizontally transmitted and MLST can be used for clinical ABA genotyping.
本研究旨在调查鲍曼不动杆菌（ABA）中耐药性与Ⅰ类、Ⅱ类和Ⅲ类整合子之间的关联。多位点序列分型（MLST）是一种用于分析ABA中管家基因簇同源性以及ABA流行情况的工具，并进一步为医院控制ABA感染提供理论依据。共收集了96株非重复ABA临床分离株，包括74株耐碳青霉烯类ABA（CRABA）和22株非CRABA菌株，用于细菌鉴定和药敏分析。对可变区进行测序和分析。然后，通过MLST扩增7对管家基因并测序，并与Pub MLST数据库进行序列比对以确定序列类型（STs）菌株并构建不同的基因型进化图。CRABAⅠ类整合子的检出率为13.51%（10/74）；未检测到Ⅱ类和Ⅲ类整合子。然而，在非CRABA菌株中未检测到Ⅰ类、Ⅱ类和Ⅲ类整合子。10个Ⅰ类整合子中的9个可变区被扩增，10个基因盒包括aacC1、aac1、aadDA1、aadA1a、aacA4、dfrA17、aadA5、aadA1、aadA22和aadA23与耐药性相关。96株ABA菌株分为21个STs：74株CRABA菌株包含9个STs，主要是ST208和ST1145，22株非CRABA菌株包含18个STs，主要是ST1145。Ⅰ类整合子是ABA耐药性的关键因素。CRABA和非CRABA菌株差异显著；前者主要包含ST208和ST1145，后者包含ST1145。大多数STs集中在重症监护病房（ICU）和神经内科，ICU的患者最易发生细菌感染。在广州医科大学附属第六医院、清远市人民医院，ABA可能存在水平传播，MLST可用于临床ABA基因分型。
