Department of Microbiology and Immunology and Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Department of Microbiology and Immunology and Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Curr Opin Virol. 2020 Oct;44:90-96. doi: 10.1016/j.coviro.2020.07.005. Epub 2020 Aug 9.
The creation of humanized mice generally involves the reconstitution of immunodeficient mice with human immune constituents. Different methodologies have been employed, and significant progress has been made towards the development of robustly humanized mouse models. Some of the techniques used include the injection of mature human immune cells, the injection of human hematopoietic stem cells (HSCs) capable of reconstituting radiation-depleted murine bone marrow, and the implantation of human fetal liver and thymus fragments under the kidney capsule to create a thymic organoid that can support thympoiesis. This review will serve as a brief introduction to the three most commonly utilized humanized mouse models for the study of gammaherpesvirus-driven pathogenesis, and highlight some of the critical discoveries these models have enabled.
人源化小鼠的构建通常涉及将人免疫成分重构到免疫缺陷小鼠中。已经采用了不同的方法学，并且在开发强大的人源化小鼠模型方面取得了重大进展。所使用的一些技术包括注射成熟的人免疫细胞、注射能够重建辐射耗尽的鼠骨髓的人造血干细胞 (HSCs)，以及将人胎肝和胸腺片段植入肾包膜下以创建能够支持胸腺发生的胸腺类器官。这篇综述将简要介绍用于研究γ疱疹病毒驱动的发病机制的三种最常用的人源化小鼠模型，并强调这些模型所带来的一些关键发现。
