Universidade Vila Velha, Vila Velha, ES, Brazil.
Rev Paul Pediatr. 2021;39:e2019165. doi: 10.1590/1984-0462/2021/39/2019165. Epub 2020 Aug 10.
To evaluate the effect of pasteurization on antioxidant and oxidant properties of human milk.
42 samples of milk before and after pasteurisation were used to evaluate the antioxidant activity by the ferric reducing capacity and by scavenging the 2,2'-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid radical. Lipid peroxidation was estimated by the concentration of malondialdehyde product using the thiobarbituric acid reactive substances assay and by the evaluation of advanced oxidation protein products.
No significant difference was observed in fresh human milk and after pasteurization in relation to antioxidant properties determined by the ferric reducing capacity (50.0±3.4% and 48.8±3.0%, respectively) and by scavenging the 2,2'-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid radical (28.9±1.5% and 31.2±1.3%, respectively). The results of malondialdehyde (62.6±4.1 and 64.3±3.6 µM/mg) and protein oxidation products (59.4±3.4 and 54.2±3.8 µM/L) of fresh and pasteurized milk, respectively, did not exhibited any significant difference.
This data showed that human milk has an important antioxidant activity and that the pasteurizing process does not influence the antioxidant capacity, avoiding the peroxidation of breast milk lipids and the formation of advanced protein oxidation products.
评估巴氏消毒对人乳抗氧化和氧化特性的影响。
使用 42 份巴氏消毒前后的牛奶样本，通过铁还原能力和清除 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基来评估抗氧化活性。通过丙二醛产物浓度（使用硫代巴比妥酸反应物质测定法）和评估高级氧化蛋白产物来评估脂质过氧化。
新鲜人乳和巴氏消毒后在铁还原能力（分别为 50.0±3.4%和 48.8±3.0%）和清除 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基（分别为 28.9±1.5%和 31.2±1.3%）方面的抗氧化特性无显著差异。新鲜和巴氏消毒牛奶的丙二醛（62.6±4.1 和 64.3±3.6 µM/mg）和蛋白氧化产物（59.4±3.4 和 54.2±3.8 µM/L）的结果也没有显著差异。
这些数据表明，人乳具有重要的抗氧化活性，巴氏消毒过程不会影响其抗氧化能力，从而避免了乳脂质的过氧化和高级蛋白氧化产物的形成。
