Bhaskaran Sreenath K, Kannappan Poornima, Muneeswari Perumalsamy, Madathil Sri R
Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore 641 021, Tamil Nadu, India.
Cardiovasc Hematol Agents Med Chem. 2021;19(2):164-174. doi: 10.2174/1871525718666200811165322.
To study the pretreatment effect of ethanolic extract of Azolla microphylla (EAM) on rat liver induced with Isoproterenol (ISO) and to identify the phytochemicals present in EAM using HPTLC and GC-MS techniques.
42 male Wistar rats were divided into 7 groups. Rats were pre-treated with EAM (250 and 500 mg/kg bw) orally for 28 days and induced with ISO (85 mg/kg; intra-peritoneal) on the 29th and 30th days. Blood and liver samples were collected from all the rats on the 30th day for biochemical and histopathological observations. HPTLC and GC-MS analyses of EAM were done using the standard protocols.
The ISO-induced group of rats displayed a significant decrease in the hepatic tissue level and activities of total protein and Aspartate Transaminase (AST), Alanine Transaminase (ALT), Alkaline Phosphatase (ALP) enzymes, respectively. Moreover, a significant decline in enzymatic and non-enzymatic antioxidants levels was spotted in the same group. However, EAM pretreatment for 28 days significantly protected the rat liver from the aforementioned alterations. Nevertheless, histopathological analysis revealed central vein dilation, necrosis, and infiltration of inflammatory cells in the ISO-induced group, wherein, EAM pretreatment significantly protected the hepatocytes from the above-mentioned changes indicating its antioxidant and cytoprotective potential. HPTLC analysis displayed the presence of flavonoids. The GC-MS analysis confirmed the presence of quercetin in EAM.
The overall results suggest that EAM pretreatment possesses an ameliorative effect against the ISO-induced oxidative damage in the rat hepatocytes.
研究小叶满江红乙醇提取物（EAM）对异丙肾上腺素（ISO）诱导的大鼠肝脏的预处理作用，并采用高效薄层色谱（HPTLC）和气相色谱-质谱联用（GC-MS）技术鉴定EAM中的植物化学成分。
将42只雄性Wistar大鼠分为7组。大鼠口服EAM（250和500 mg/kg体重）预处理28天，并在第29天和第30天腹腔注射ISO（85 mg/kg）。在第30天从所有大鼠采集血液和肝脏样本，进行生化和组织病理学观察。EAM的HPTLC和GC-MS分析按照标准方案进行。
ISO诱导组大鼠的肝组织中总蛋白水平以及天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）的活性均显著降低。此外，该组的酶促和非酶促抗氧化剂水平也显著下降。然而，EAM预处理28天显著保护大鼠肝脏免受上述改变的影响。尽管如此，组织病理学分析显示ISO诱导组出现中央静脉扩张、坏死和炎性细胞浸润，其中EAM预处理显著保护肝细胞免受上述变化影响，表明其具有抗氧化和细胞保护潜力。HPTLC分析显示存在黄酮类化合物。GC-MS分析证实EAM中存在槲皮素。
总体结果表明，EAM预处理对ISO诱导的大鼠肝细胞氧化损伤具有改善作用。
