产油酵母罗伦隐球酵母的完整线粒体基因组。

The complete mitochondrial genome of the lipid-producing yeast Rhodotorula toruloides.

机构信息

Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.

School of Chemical Engineering, University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing 100049, China.

出版信息

FEMS Yeast Res. 2020 Sep 1;20(6). doi: 10.1093/femsyr/foaa048.

DOI:10.1093/femsyr/foaa048

PMID:32789504

Abstract

Mitochondria are semi-autonomous organelles with their own genome and crucial to cellular material and energy metabolism. Here, we report the complete mitochondrial genome of a lipid-producing basidiomycetous yeast Rhodotorula toruloides NP11. The mitochondrial genome of R. toruloides NP11 was assembled into a circular DNA molecule of 125937bp, encoding 15 proteins, 28 transfer RNAs, 2 ribosomal RNA subunits and 10 open reading frames with unknown function. The G + C content (41%) of the mitochondrial genome is substantially lower than that of the nuclear genome (62%) of R. toruloides NP11. Further reanalysis of the transcriptome data confirmed the transcription of four mitochondrial genes. The comparison of the mitochondrial genomes of R. toruloides NP11 and NBRC0880 revealed a significant genetic divergence. These data can complement our understanding of the genetic background of R. toruloides and provide fundamental information for further genetic engineering of this strain.

摘要

线粒体是具有自身基因组的半自主细胞器，对细胞物质和能量代谢至关重要。在这里，我们报道了产脂担子菌红酵母 NP11 的完整线粒体基因组。红酵母 NP11 的线粒体基因组组装成一个 125937bp 的圆形 DNA 分子，编码 15 种蛋白质、28 种转移 RNA、2 个核糖体 RNA 亚基和 10 个具有未知功能的开放阅读框。线粒体基因组的 G+C 含量（41%）明显低于红酵母 NP11 的核基因组（62%）。对转录组数据的进一步分析证实了 4 个线粒体基因的转录。红酵母 NP11 和 NBRC0880 的线粒体基因组比较显示出显著的遗传分化。这些数据可以补充我们对红酵母 NP11 遗传背景的理解，并为该菌株的进一步遗传工程提供基础信息。