用于制备庆大霉素腺嘌呤单核苷酸转移酶以进行庆大霉素酶促测定的双渗透休克程序。
Double-osmotic-shock procedure to prepare gentamicin adenine mononucleotide transferase for the enzymatic assay of gentamicin.
作者信息
Dankert J, Woltjes J
出版信息
Antimicrob Agents Chemother. 1977 Jun;11(6):1074-6. doi: 10.1128/AAC.11.6.1074.
Abstract
The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.
摘要
在对大肠杆菌K-12 W677/HJR66进行单次冷渗透休克处理过程中，庆大霉素腺嘌呤单核苷酸转移酶的释放并非总是达到最大值。当采用双休克程序时，渗透提取物中庆大霉素腺嘌呤单核苷酸转移酶的量会显著增加。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
R-factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics.R 因子介导的庆大霉素耐药性：一种修饰氨基糖苷类抗生素的新酶。
FEBS Lett. 1971 May 20;14(5):293-296. doi: 10.1016/0014-5793(71)80282-x.
3
Enzymatic assay for gentamicin.
N Engl J Med. 1973 Jan 11;288(2):108. doi: 10.1056/nejm197301112880223.
4
Enzymatic assay for gentamicin and related aminoglycoside antibiotics.
J Infect Dis. 1974 May;129(5):519-27. doi: 10.1093/infdis/129.5.519.
5
Assay for gentamicin.
N Engl J Med. 1972 Nov 23;287(21):1100. doi: 10.1056/nejm197211232872122.
6
A rapid chemical assay for gentamicin.庆大霉素的快速化学检测法。
N Engl J Med. 1972 Mar 16;286(11):583-6. doi: 10.1056/NEJM197203162861106.
7
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8
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J Biol Chem. 1966 Jul 10;241(13):3055-62.
9
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