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用于制备庆大霉素腺嘌呤单核苷酸转移酶以进行庆大霉素酶促测定的双渗透休克程序。

Double-osmotic-shock procedure to prepare gentamicin adenine mononucleotide transferase for the enzymatic assay of gentamicin.

作者信息

Dankert J, Woltjes J

出版信息

Antimicrob Agents Chemother. 1977 Jun;11(6):1074-6. doi: 10.1128/AAC.11.6.1074.

DOI:10.1128/AAC.11.6.1074
PMID:327923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC352133/
Abstract

The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.

摘要

在对大肠杆菌K-12 W677/HJR66进行单次冷渗透休克处理过程中，庆大霉素腺嘌呤单核苷酸转移酶的释放并非总是达到最大值。当采用双休克程序时，渗透提取物中庆大霉素腺嘌呤单核苷酸转移酶的量会显著增加。

相似文献

1
Double-osmotic-shock procedure to prepare gentamicin adenine mononucleotide transferase for the enzymatic assay of gentamicin.用于制备庆大霉素腺嘌呤单核苷酸转移酶以进行庆大霉素酶促测定的双渗透休克程序。
Antimicrob Agents Chemother. 1977 Jun;11(6):1074-6. doi: 10.1128/AAC.11.6.1074.
2
Gentamicin assay by enzymatic adenylylation and the application of a double osmotic shock procedure to prepare gentamicin adenine mono-nucleotide transferase.通过酶促腺苷酸化法测定庆大霉素，并应用双重渗透压休克程序制备庆大霉素腺嘌呤单核苷酸转移酶。
Zentralbl Bakteriol Orig A. 1977 Sep;239(1):113-23.
3
Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin.庆大霉素：腺嘌呤单核苷酸转移酶：部分纯化、特性鉴定及其在庆大霉素临床定量分析中的应用
J Infect Dis. 1974 Apr;129(4):391-401. doi: 10.1093/infdis/129.4.391.
4
Preparation of stable gentamicin adenylyl transferase of high specific activity.
Biochem Biophys Res Commun. 1975 Oct 27;66(4):1408-13. doi: 10.1016/0006-291x(75)90516-1.
5
Properties of gentamicin adenylyl transferase obtained from R-factor-resistant Escherichia coli.从抗R因子的大肠杆菌中获得的庆大霉素腺苷酸转移酶的特性
Int J Biochem. 1980;12(3):427-31. doi: 10.1016/0020-711x(80)90123-8.
6
Purification and properties of gentamicin nucleotidyltransferase from Escherichia coli: nucleotide specificity, pH optimum, and the separation of two electrophoretic variants.大肠杆菌庆大霉素核苷酸转移酶的纯化及性质：核苷酸特异性、最适pH值以及两种电泳变体的分离
Arch Biochem Biophys. 1984 Apr;230(1):250-63. doi: 10.1016/0003-9861(84)90106-1.
7
Rapid gentamicin assay by enzymatic adenylylation.通过酶促腺苷化进行庆大霉素快速检测。
J Clin Pathol. 1974 Jun;27(6):452-6. doi: 10.1136/jcp.27.6.452.
8
Gentamicin-adenylyltransferase activity as a cause of gentamicin resistance in clinical isolates of Pseudomonas aeruginosa.庆大霉素腺苷酸转移酶活性是铜绿假单胞菌临床分离株中庆大霉素耐药的一个原因。
Antimicrob Agents Chemother. 1974 Jun;5(6):565-70. doi: 10.1128/AAC.5.6.565.
9
Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence.
Nat New Biol. 1972 Aug 23;238(86):233-4. doi: 10.1038/newbio238233a0.
10
Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa.
J Antibiot (Tokyo). 1982 Feb;35(2):235-44. doi: 10.7164/antibiotics.35.235.

引用本文的文献

1
Relation between aminoglycoside 2"-O-nucleotidyltransferase activity and aminoglycoside resistance.氨基糖苷2”-O-核苷酸基转移酶活性与氨基糖苷耐药性之间的关系
Antimicrob Agents Chemother. 1984 Feb;25(2):234-7. doi: 10.1128/AAC.25.2.234.
2
Aminoglycoside phosphotransferase-II-mediated amikacin resistance in Escherichia coli.大肠杆菌中氨基糖苷磷酸转移酶-II介导的阿米卡星耐药性。
Antimicrob Agents Chemother. 1981 Sep;20(3):344-50. doi: 10.1128/AAC.20.3.344.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
R-factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics.R 因子介导的庆大霉素耐药性：一种修饰氨基糖苷类抗生素的新酶。
FEBS Lett. 1971 May 20;14(5):293-296. doi: 10.1016/0014-5793(71)80282-x.
3
Enzymatic assay for gentamicin.庆大霉素的酶法测定
N Engl J Med. 1973 Jan 11;288(2):108. doi: 10.1056/nejm197301112880223.
4
Enzymatic assay for gentamicin and related aminoglycoside antibiotics.
J Infect Dis. 1974 May;129(5):519-27. doi: 10.1093/infdis/129.5.519.
5
Assay for gentamicin.庆大霉素测定
N Engl J Med. 1972 Nov 23;287(21):1100. doi: 10.1056/nejm197211232872122.
6
A rapid chemical assay for gentamicin.庆大霉素的快速化学检测法。
N Engl J Med. 1972 Mar 16;286(11):583-6. doi: 10.1056/NEJM197203162861106.
7
Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin.庆大霉素：腺嘌呤单核苷酸转移酶：部分纯化、特性鉴定及其在庆大霉素临床定量分析中的应用
J Infect Dis. 1974 Apr;129(4):391-401. doi: 10.1093/infdis/129.4.391.
8
The release of enzymes by osmotic shock from Escherichia coli in exponential phase.处于对数期的大肠杆菌通过渗透休克释放酶。
J Biol Chem. 1966 Jul 10;241(13):3055-62.
9
Preparation of stable gentamicin adenylyl transferase of high specific activity.
Biochem Biophys Res Commun. 1975 Oct 27;66(4):1408-13. doi: 10.1016/0006-291x(75)90516-1.