通过酶促腺苷化进行庆大霉素快速检测。
Rapid gentamicin assay by enzymatic adenylylation.
作者信息
ten Krooden E, Darrell J H
出版信息
J Clin Pathol. 1974 Jun;27(6):452-6. doi: 10.1136/jcp.27.6.452.
Abstract
A method is described for the assay of gentamicin using the enzyme gentamicin adenylyltransferase derived from an R-factor-carrying strain of Escherichia coli. The reaction involves adenylylation of the gentamicin with ((14)C)-ATP to form a radioactively labelled product. The technique is compared with the plate diffusion and the urease methods. The adenylylation technique gives results comparable to the plate diffusion assay and is more accurate than the urease method.
摘要
描述了一种使用源自携带R因子的大肠杆菌菌株的庆大霉素腺苷酸转移酶来测定庆大霉素的方法。该反应涉及庆大霉素与（14C）-ATP进行腺苷酸化反应，以形成放射性标记产物。将该技术与平板扩散法和脲酶法进行了比较。腺苷酸化技术得到的结果与平板扩散法相当，且比脲酶法更准确。
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本文引用的文献
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FEBS Lett. 1971 May 20;14(5):293-296. doi: 10.1016/0014-5793(71)80282-x.
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Enzymatic assay for gentamicin.
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Mechanisms of antibiotic resistance in bacteria.细菌中的抗生素耐药机制。
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A rapid chemical assay for gentamicin.庆大霉素的快速化学检测法。
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Med J Aust. 1973 Jul 21;2(3):114-6. doi: 10.5694/j.1326-5377.1973.tb128693.x.
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Science. 1967 Jun 16;156(3781):1451-5. doi: 10.1126/science.156.3781.1451.
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The release of enzymes by osmotic shock from Escherichia coli in exponential phase.处于对数期的大肠杆菌通过渗透休克释放酶。
J Biol Chem. 1966 Jul 10;241(13):3055-62.
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