Cheng L, Binder S W, Fu Y S, Lewin K J
Department of Pathology, University of California, Los Angeles.
Lab Invest. 1988 Mar;58(3):346-53.
An immunohistochemical technique using a commercially available monoclonal antibody to the estrogen receptor in formalin-fixed breast tumors is described. The author's technique is compared to three other recently published techniques in 27 case studies. The authors' method using pronase enzyme pretreatment and alkaline phosphatase as the third antibody yielded the best results. Comparison with the standard dextran-coated charcoal cytosolic assay results from the cases selected yielded a 100% sensitivity and 89% specificity for the authors' technique. The advantages of the immunohistochemical method over the biochemical assay are discussed and clinical implications are suggested. A step-by-step procedure for the authors' technique follows the text.
本文描述了一种在福尔马林固定的乳腺肿瘤中使用市售雌激素受体单克隆抗体的免疫组织化学技术。在27个病例研究中，将作者的技术与其他三种最近发表的技术进行了比较。作者使用链霉蛋白酶酶预处理和碱性磷酸酶作为第三抗体的方法得出了最佳结果。对于所选病例，将作者的技术与标准葡聚糖包被活性炭胞质测定结果进行比较，得到其敏感性为100%，特异性为89%。文中讨论了免疫组织化学方法相对于生化测定的优势，并提出了临床意义。作者技术的一步步操作步骤紧跟正文之后。
