DeRosa C M, Ozzello L, Habif D V, Konrath J G, Greene G L
Arthur Purdy Stout Laboratory of Surgical Pathology, Columbia University, New York.
Ann Surg. 1989 Aug;210(2):224-8. doi: 10.1097/00000658-198908000-00015.
A peroxidase-antiperoxidase technique was used to visualize estrogen and progesterone receptors in stored imprints and cryostat sections of breast carcinomas that were prepared at the time of biopsy or frozen section diagnosis. This was done to provide an alternate technique for the assessment of the receptor status of tumors that could not be adequately assayed with other biochemical or immunocytological methods. Fixation in Zamboni's fixative followed by passage through cold methanol and acetone before storage at -80 C insured good preservation of the receptor proteins over extended periods of time (up to 56 weeks). Immunostaining of these stored preparations with monoclonal antibodies against estrogen receptor (H222) and progesterone receptor (JZB39 and KD68) showed a high degree of correspondence with immunocytochemical assays (ER-ICA and PR-ICA) and biochemical analysis. This technique is easy to perform and provides reliable information, even in tumors that are too small and/or ill defined to permit separate sampling for receptor assays.
采用过氧化物酶-抗过氧化物酶技术，对活检或冷冻切片诊断时制备的乳腺癌存档印片和冷冻切片中的雌激素和孕激素受体进行可视化检测。这样做是为了提供一种替代技术，用于评估那些无法用其他生化或免疫细胞方法进行充分检测的肿瘤的受体状态。用赞博尼固定液固定，然后依次通过冷甲醇和丙酮，再于-80℃保存，可确保受体蛋白在较长时间内（长达56周）得到良好保存。用抗雌激素受体单克隆抗体（H222）和抗孕激素受体单克隆抗体（JZB39和KD68）对这些存档标本进行免疫染色，结果显示与免疫细胞化学检测（ER-ICA和PR-ICA）及生化分析具有高度一致性。该技术操作简便，即使对于过小和/或边界不清而无法单独取样进行受体检测的肿瘤，也能提供可靠信息。
