两种用于水生细菌计数的直接计数技术的比较。
Comparison of two direct-count techniques for enumerating aquatic bacteria.
作者信息
Bowden W B
出版信息
Appl Environ Microbiol. 1977 May;33(5):1229-32. doi: 10.1128/aem.33.5.1229-1232.1977.
Abstract
Planktonic bacteria from an estuary were concentrated on membrane filters and counted with both a scanning electron microscope and an epi-illuminated fluorescent microscope. Counts on 0.2 micron Nuclepore filters (polycarbonate) were significantly higher (P less than 0.001) than counts on 0.2-micron Sartorius filters (cellulose). In contrast, there was not a statistically significant difference between the two techniques when Nuclepore filters were used (0.5 less than P less than 0.9). The average cell volume from this study area was 0.047 micron3. The estimated number of bacteria ranged from 10(6) to 10(7) bacteria per ml, representing from 4 to 40 mg of C per m3.
摘要
河口的浮游细菌被浓缩在膜滤器上，并用扫描电子显微镜和落射荧光显微镜进行计数。在0.2微米的核孔滤膜（聚碳酸酯）上的计数显著高于（P小于0.001）在0.2微米的赛多利斯滤膜（纤维素）上的计数。相比之下，当使用核孔滤膜时，两种技术之间没有统计学上的显著差异（0.5小于P小于0.9）。该研究区域的细菌平均细胞体积为0.047立方微米。估计每毫升细菌数量在10⁶至10⁷个之间，相当于每立方米4至40毫克碳。
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