Manisa Celal Bayar University, Faculty of Medicine, Department of Otorhinolaryngology-Head Neck Surgery, Manisa, Turkey.
Manisa Celal Bayar University, Faculty of Science and Letters, Department of Biology, Manisa, Turkey.
Braz J Otorhinolaryngol. 2022 Jul-Aug;88(4):505-510. doi: 10.1016/j.bjorl.2020.06.015. Epub 2020 Aug 1.
Pregnancy rhinitis is a common sex hormone-related otorhinolaryngological disorder. There are some epidemiological and physiological studies on pregnancy rhinitis, but histopathological and biomolecular changes have not been studied thoroughly.
The receptors VPAC1 and VPAC2 are known for their roles in allergic rhinitis. On the other hand, activation of subclinical allergy has been suggested in the pathophysiology of pregnancy rhinitis. Therefore, we aimed to compare the physiological and gestational pattern of VPAC1 and VPAC2 expression in rat nasal mucosa.
Twenty adult Wister albino female rats were enrolled into the study. Two groups constituted as 10 control (group A) and 10 pregnant (group B) rats. They were fed ad libitum and sheltered at room temperature (22°±2°C). The rats were sacrificed at the 20th day of gestation by intraperitoneal injection of 400mg/kg Na-pentobarbitone. Then, 10-15mL of blood was taken, and samples were reserved for the detection of serum estradiol and progesterone levels by ELISA test. The nasal septum was resected and divided in half for immunohistochemical analyses and real time polymerase chain reaction testing of VPAC1 and VPAC2.
VPAC1 and VPAC2 were found to be in all layers of septal specimens, but the immunostaining of surface epithelium was more distinct in specimens of both groups. We demonstrated higher overall staining intensity in the pregnant group. PCR revealed significant increase in expression of VPAC1 (p=0.023) and VPAC2 (p=0.021) in pregnant group when compared with control group. In addition, we demonstrated upregulatory effect of estradiol and progesterone on the vasoactive intestinal peptide receptor expression.
Gestational up-regulation of nasal VPAC1 and VPAC2 was shown both by PCR and immunohistochemical analysis. These findings support the hypothesis that PR is caused by the activation of subclinical allergy that is present before pregnancy.
妊娠性鼻炎是一种常见的与性激素相关的耳鼻喉科疾病。虽然有一些关于妊娠性鼻炎的流行病学和生理学研究，但对其组织病理学和生物分子变化尚未进行深入研究。
已知 VPAC1 和 VPAC2 受体在过敏性鼻炎中起作用。另一方面，在妊娠性鼻炎的病理生理学中，已提出亚临床过敏的激活。因此，我们旨在比较大鼠鼻黏膜中 VPAC1 和 VPAC2 表达的生理和妊娠模式。
将 20 只成年 Wister 白化雌性大鼠纳入研究。将它们分为两组，每组 10 只，分别为对照组（A 组）和妊娠组（B 组）。两组大鼠均自由进食，在室温（22°±2°C）下饲养。妊娠第 20 天，通过腹腔注射 400mg/kg 的戊巴比妥钠处死大鼠。然后，抽取 10-15mL 血液，通过 ELISA 试验检测血清雌二醇和孕酮水平。切除鼻中隔并将其分为两半，用于 VPAC1 和 VPAC2 的免疫组织化学分析和实时聚合酶链反应检测。
VPAC1 和 VPAC2 均存在于鼻中隔标本的所有层中，但两组标本的表面上皮免疫染色更为明显。我们发现妊娠组的整体染色强度更高。PCR 显示，与对照组相比，妊娠组 VPAC1（p=0.023）和 VPAC2（p=0.021）的表达均显著增加。此外，我们还证明了雌二醇和孕酮对血管活性肠肽受体表达的上调作用。
PCR 和免疫组织化学分析均显示妊娠时鼻 VPAC1 和 VPAC2 的表达上调。这些发现支持妊娠性鼻炎是由妊娠前存在的亚临床过敏激活引起的假说。
