Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2-nocho, Nishi, Niigata, 950-2181, Japan.
Anal Sci. 2020 Dec 10;36(12):1495-1499. doi: 10.2116/analsci.20P235. Epub 2020 Aug 14.
Several scientific works have reported the use of colloidal gold nanoparticle (AuNP) solutions as a colorimetric probe for creatinine detection. Nonetheless, urinary protein is one of the primary chemical components that can interfere with creatinine detection. In this work, we developed a colorimetric probe using AuNP colloidal solution to detect creatinine in the urine of proteinuria patients. A microchamber array was prepared to minimize the sample volume and was used to simultaneously perform spectral recording and image acquisition of several samples. The analyzed volume for each sample was 15 μL. A camera coupled with liquid crystal tunable filters was used to record hyperspectral images, and the signals were then converted to localized surface plasmon resonance (LSPR) spectra. Color changes in the AuNP colloidal solution in the presence of varying concentrations of creatinine and human serum albumin (HSA) indicated different features and could be detected by a hyperspectral imaging technique. The relevant concentration ranges of creatinine and HSA were 5 - 200 and 50 - 250 mg dL, respectively. Furthermore, a smartphone camera was adopted to record a color mapping image of the AuNP colloidal solution in the presence of creatinine and HSA at these concentration ranges. Contour plots of red and blue chromaticity levels from color mappings were produced, and 2D fitting equations obtained from these contour plots were adopted to determine the creatinine concentration in the urine of proteinuria patients. This practical technique can be used for screening and can be further developed as a household biosensing device for urinalysis.
已有几项科学研究报告称，胶体金纳米粒子（AuNP）溶液可作为肌酐检测的比色探针。然而，尿液蛋白是可能干扰肌酐检测的主要化学成分之一。在这项工作中，我们开发了一种使用 AuNP 胶体溶液的比色探针，用于检测蛋白尿患者尿液中的肌酐。制备了微腔阵列以最小化样品体积，并用于同时对多个样品进行光谱记录和图像采集。每个样品的分析体积为 15 μL。用耦合有液晶可调滤波器的相机记录高光谱图像，然后将信号转换为局域表面等离子体共振（LSPR）光谱。在存在不同浓度肌酐和人血清白蛋白（HSA）的情况下，AuNP 胶体溶液的颜色变化显示出不同的特征，可以通过高光谱成像技术检测到。肌酐和 HSA 的相关浓度范围分别为 5-200 和 50-250 mg dL。此外，采用智能手机相机记录了在这些浓度范围内存在肌酐和 HSA 的 AuNP 胶体溶液的颜色映射图像。生成了颜色映射的红色和蓝色色度级别的等高线图，并采用这些等高线图获得的 2D 拟合方程来确定蛋白尿患者尿液中的肌酐浓度。这项实用技术可用于筛查，并可进一步开发为家用尿液分析生物传感装置。
