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LINC00501通过介导miR-129-5p/HMGB1抑制肺癌的生长和转移。

LINC00501 Inhibits the Growth and Metastasis of Lung Cancer by Mediating miR-129-5p/HMGB1.

作者信息

Wang Ruo-Tian, Zhang Yi, Yao Shu-Yang, Tan Xiao-Gang

机构信息

Department of Thoracic Surgery, Xuanwu Hospital Capital Medical University, Beijing 100053, People's Republic of China.

出版信息

Onco Targets Ther. 2020 Jul 24;13:7137-7149. doi: 10.2147/OTT.S254735. eCollection 2020.

DOI:10.2147/OTT.S254735
PMID:32801746
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7386808/
Abstract

BACKGROUND

The function of LINC00501, a long-non-coding RNA (lncRNA), is unclear at present. According to the Cancer Genome Atlas (TCGA), LINC00501 is highly expressed in lung cancer (LC), but whether it can be adopted as a potential therapy target for LC still needs further research.

METHODS

The expression of LINC00501 in LC was analyzed based on the TCGA, and a real-time fluorescent quantitative PCR assay was carried out to quantify LINC00501 in non-small-cell lung cancer (NSCLC). Additionally, bioinformatics analysis, luciferase reporter gene technique, and RNA immunoprecipitation (RIP) were employed to analyze the direct interaction between LINC00501 and miR-129-5p, and CCK-8 and Transwell assays and flow cytometry were employed to analyze the effects of LINC00501 on cell proliferation, invasion, and apoptosis. Furthermore, a Western blot assay was carried out to determine the protein level of HMGB1.

RESULTS

LINC00501 was highly expressed in LC according to the database, and it was found that LINC00501 was upregulated in NSCLC specimens and cells, and the up-regulation indicated an unfavorable prognosis. Besides, knockdown of LINC00501 hindered the proliferation and invasion of NSCLC cells and intensified their apoptosis, and LINC00501 could be adopted as competitive endogenous RNA to regulate HMGB1 and tumorigenesis through miR-129-5p.

CONCLUSION

LINC00501 is overexpressed in LC and the overexpression indicates poor prognosis of patients. In addition, LINC00501 can inhibit the invasion and migration of LC by mediating miR-129-5p/HMGB1.

摘要

背景

长链非编码RNA（lncRNA）LINC00501的功能目前尚不清楚。根据癌症基因组图谱（TCGA），LINC00501在肺癌（LC）中高表达，但它是否可作为LC的潜在治疗靶点仍需进一步研究。

方法

基于TCGA分析LINC00501在LC中的表达，并进行实时荧光定量PCR检测以定量非小细胞肺癌（NSCLC）中的LINC00501。此外，采用生物信息学分析、荧光素酶报告基因技术和RNA免疫沉淀（RIP）分析LINC00501与miR-129-5p之间的直接相互作用，并采用CCK-8和Transwell检测以及流式细胞术分析LINC00501对细胞增殖、侵袭和凋亡的影响。此外，进行蛋白质免疫印迹检测以确定HMGB1的蛋白水平。

结果

根据数据库，LINC00501在LC中高表达，发现LINC00501在NSCLC标本和细胞中上调，且上调表明预后不良。此外，敲低LINC00501可抑制NSCLC细胞的增殖和侵袭并增强其凋亡，且LINC00501可作为竞争性内源性RNA通过miR-129-5p调节HMGB1和肿瘤发生。

结论

LINC00501在LC中过表达，且过表达表明患者预后不良。此外，LINC00501可通过介导miR-129-5p/HMGB1抑制LC的侵袭和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/293656cad2ce/OTT-13-7137-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/cb3d2f1aed91/OTT-13-7137-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/2f4585108de4/OTT-13-7137-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/e44e4d95bb56/OTT-13-7137-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/a74ffacfd901/OTT-13-7137-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/2ac2b3a3e7c0/OTT-13-7137-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/891c70bad995/OTT-13-7137-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/293656cad2ce/OTT-13-7137-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/cb3d2f1aed91/OTT-13-7137-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/2f4585108de4/OTT-13-7137-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/e44e4d95bb56/OTT-13-7137-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/a74ffacfd901/OTT-13-7137-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/2ac2b3a3e7c0/OTT-13-7137-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/891c70bad995/OTT-13-7137-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7b8/7386808/293656cad2ce/OTT-13-7137-g0007.jpg

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