长链非编码 RNA CASC11 促进 TGF-β1，增加小细胞肺癌中癌细胞的干性并预测术后生存。
LncRNA CASC11 promotes TGF-β1, increases cancer cell stemness and predicts postoperative survival in small cell lung cancer.
机构信息
Department of Oncology, Liaocheng People's Hospital, Liaocheng City, Shandong Province 252000, PR China.
Department of Children Healthcare, Liaocheng People's Hospital, Liaocheng City, Shandong Province 252000, PR China.
出版信息
Gene. 2019 Jul 1;704:91-96. doi: 10.1016/j.gene.2019.04.019. Epub 2019 Apr 6.
LncRNA CASC11 is a recently identified oncogenic lncRNA in colorectal cancer. This study aimed to investigate the role of lncRNA CASC11 in small cell lung cancer (SCLC). In the present study, expression levels of CASC11 and TGF-β1 were found to be positively and significantly correlated with the percentage of CDD133+ cells of SCLC cell lines. Plasma CASC11 and TGF-β1 were upregulated and positively correlated in SCLC patients, but not in healthy controls. Upregulation of plasma CASC11 and TGF-β1 predicted poor survival of SCLC patients. Overexpression of CASC11 and TGF-β1 also resulted in the increased percentage of CDD133+ cells of SCLC cell lines, while TGF-β inhibitor attenuated the effects of CASC11 overexpression. CASC11 overexpression mediated the upregulation of TGF-β1 in SCLC cells, while treatment with exogenous TGF-β1 showed no significant effect on CASC11. Therefore, lncRNA CASC11 promotes TGF-β1, increases cancer cell stemness and predicts postoperative survival in SCLC.
长链非编码 RNA CASC11 是结直肠癌中最近鉴定的一种致癌长链非编码 RNA。本研究旨在探讨长链非编码 RNA CASC11 在小细胞肺癌（SCLC）中的作用。在本研究中，发现 CASC11 和 TGF-β1 的表达水平与 SCLC 细胞系中 CDD133+细胞的百分比呈正相关且显著相关。SCLC 患者的血浆 CASC11 和 TGF-β1 上调且呈正相关，但在健康对照者中则无。血浆 CASC11 和 TGF-β1 的上调预测 SCLC 患者的生存不良。CASC11 和 TGF-β1 的过表达也导致 SCLC 细胞系中 CDD133+细胞的百分比增加，而 TGF-β 抑制剂减弱了 CASC11 过表达的作用。CASC11 过表达介导 SCLC 细胞中 TGF-β1 的上调，而外源性 TGF-β1 处理对 CASC11 无明显影响。因此，长链非编码 RNA CASC11 促进 TGF-β1，增加肿瘤细胞干性，并预测 SCLC 的术后生存。
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